癌-睾丸抗原基因在神经胶质瘤干细胞中的表达

背景：癌-睾丸抗原基因在胶质瘤干细胞表达还不甚清楚。 目的：检测癌-睾丸抗原基因在神经胶质瘤干细胞中的表达。 方法：通过神经球培养的方法而分离干细胞。以半定量PCR及实时荧光定量PCR检测癌-睾丸抗原基因在母细胞、干细胞与分化细胞中的表达。 结果与结论：以神经球培养方式从胶质瘤细胞系中可分离出肿瘤球,将肿瘤球培养在含血清的培养基中,它的形态呈贴壁分化,且与母细胞没有区别。半定量PCR及实时荧光定量PCR检测发现与母细胞和分化细胞相比,癌-睾丸抗原基因在干细胞中的表达最高。提示癌-睾丸抗原基因可能为肿瘤干细胞的表面抗原。

Expression of cancer-testis antigen in glioma stem cells

BACKGROUND: It is still unclear on the expression of cancer-testis antigen genes in glioma stem cells. OBJECTIVE:To detect the expression of cancer testis antigen genes in glioma stem cells. METHODS:Stem cells were cultured and isolated by neurosphere culture methods in DMEM/F12. Then the expression of cancer testis antigen genes were analyzed among parent cells, stem cells and differentiated cells by using semiquantitative RT-PCR and real time PCR. RESULTS AND CONCLUSION:We isolated tumor spheres from glioma cell lines by neurosphere culture. And then tumor spheres were cultured in medium containing serum, the morphology of the tumor spheres changed to adherent and they were not distinguishable from the parent cells. The expression of cancer testis antigen genes were analyzed by semiquantitative RT-PCR and quantitative RT-PCR and it was found that the expression were highest in stem cells compared with parent cells and differentiated cells. Cancer testis antigen genes may present as surface antigens in cancer stem cells and these findings suggest that cancer testis antigen genes may be helpful for targeted vaccine therapy against cancer stem cells in glioma patients.