静脉输注过氧化氢溶液上调小鼠脾脏和外周血CD4+Foxo3+调节性T细胞

背景：现已证实，天然生成的调节性T细胞在维护机体免疫稳态、抑制炎性反应、抗移植排斥、抑制抗肿瘤免疫应答以及防治自身免疫病中都有重要的功能和作用。 目的：探讨静脉输注过氧化氢对BALB/c小鼠CD4⁺CD25⁺Foxo3⁺ 调节性T细胞的影响。 方法：取BALB/c小鼠12只,随机分为两组。H₂O₂处理组小鼠尾静脉注射含有H₂O₂的PBS；PBS组小鼠静脉注射无菌PBS。分别在注射2周后处死小鼠，采集小鼠外周血、脾脏和胸腺，采用流式细胞仪检测其中CD4⁺CD25⁺ Foxp3⁺T细胞比例。 结果与结论：H₂O₂处理组小鼠外周血和脾脏CD4⁺Foxp3⁺ T细胞比例、CD4⁺Foxp3⁺/CD4⁺细胞比例均高于对照组小鼠(P < 0.05)。提示静脉注射H₂O₂能上调小鼠外周血和脾脏内CD4⁺Foxp3⁺调节性T细胞的比例。

Intravenous infusion of hydrogen peroxide upregulates CD4+Foxo3+ regulatory T cells in mouse spleen and peripheral blood

BACKGROUND: The naturally formed regulator T cells have been confirmed exert important function and role in maintaining organism’s homeostasis, inhibiting inflammation, prevent against transplantation rejection, inhibiting anti-tumor immune response, and preventing and treating auto-immune diseases. OBJECTIVE:To investigate the effects of intravenous infusion of hydrogen peroxide (H₂O₂) on CD4⁺CD25⁺Foxo3⁺ regulatory T cells in the BALB/c mice. METHODS: Twelve male BALB/c mice of specific pathogen free level, 6 weeks old, were randomly divided into two groups, H₂O₂ treatment group and PBS group. The mice in the H₂O₂ treatment group were injected 250 μL PBS with 50 mmol/L H2O2 via tail vein. The mice in the PBS group were injected 250 μL PBS via tail vein. At 2 weeks post injection, all mice were killed to collect peripheral blood, spleen and thymus. Flow cytometry was used to detect the proportion of CD4⁺CD25⁺Foxp3 T cells. RESULTS AND CONCLUSION:The proportion of CD4⁺Foxp3⁺ T cells and CD4⁺Foxp3⁺/CD4⁺ cells in the peripheral blood and spleen of mice in the H₂O₂ treatment group was significantly higher than that in the PBS group (P < 0.05). These findings suggest that intravenous injection of H₂O₂ can increase the proportion of CD4⁺Foxp3⁺ regulatory T cells in the mouse peripheral blood and spleen.