脑缺血再灌注损伤模型大鼠参附注射液干预后热休克蛋白70的表达 |
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作者姓名: | 江承平 刘 福 李 毅 王柏强 唐晓蓉 吴碧华 王晓明 |
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作者单位: | 1川北医学院附属医院药剂科,四川省南充市 637007
2川北医学院神经病学研究所,四川省南充市 637000 |
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基金项目: | 四川省科技厅资助项目 (2010JY0094),课题名称:参附注射液对实验性脑缺血再灌注损伤保护作用及其机制。 |
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摘 要: | 背景:参附注射液可通过改善微循环,增加组织血氧含量发挥对缺血再灌注损伤的保护作用。
目的:观察参附注射液对大鼠脑缺血再灌注损伤后热休克蛋白70表达的影响。
方法:将SD大鼠随机分为3组:假手术组、大脑中动脉闭塞缺血再灌注损伤模型组、参附注射液组。
结果与结论:应用参附注射液1 d后,改善了缺血再灌注大鼠脑部神经细胞的排列,减轻了胞体肿胀,核固缩等现象,应用3 d后改善更为明显,胞体结构已较清晰,核固缩、溶解程度显著减轻,胞体肿胀现象明显改善。参附注射液组治疗后1,3 d热休克蛋白70表达明显高于假手术组与模型组。提示参附注射液对脑缺血再灌注具有显著的保护作用,其作用可能是通过促进热休克蛋白70的表达来实现的。
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关 键 词: | 参附注射液 缺血再灌注损伤 热休克蛋白70 脑 大鼠 |
收稿时间: | 2011-07-07 |
Heat shock protein 70 expression after Shenfu injection administration in a rat model of cerebral ischemia/reperfusion injury |
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Authors: | Jiang Cheng-ping Liu Fu Li Yi Wang Bai-qiang Tang Xiao-rong Wu Bi-hua Wang Xiao-ming |
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Institution: | 1Department of Pharmacology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637007, Sichuan Province, China
2Neurology Research Institute, North Sichuan Medical College, Nanchong 637007, Sichuan Province, China |
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Abstract: | BACKGROUND:Shenfu injection plays a protective effect on ischemia/reperfusion injury in improving microcirculation and increasing blood oxygen content in tissue.
OBJECTIVE:To investigate the effects of Shenfu injection on heat shock protein 70 (Hsp70) expression in a rat model of cerebral ischemia/reperfusion injury.
METHODS:SD rats were randomly divided into three groups (n=32 for each group): sham-operated, middle cerebral artery occlusion (MCAO), and Shenfu injection.
RESULTS AND CONCLUSION:Shenfu injection improved the arrangement of brain nerve cells, reduced the swelling of cell bodies and pyknosis at 1 day after administration. At 3 days after Shenfu injection administration, a more obvious trend on the attenuation of the above phenomena was observed. At 1 and 3 days, Hsp70 expression was significantly higher in the Shenfu injection group than in the sham-operated and MCAO groups. These finding suggest that Shenfu injection exhibits a significant protective effect on cerebral ischemia/reperfusion injury, and this protective effect may be implemented by increasing Hsp70 expression. |
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