链霉素缓释复合降解膜植入损伤肌腱局部腱周结缔组织的变化

背景：国内外学者曾用生物、非生物及药物等方法,诸如生物膜、透明质酸钠、纤维素密封胶等材料进行大量肌腱修复防粘连研究,但到目前为止尚未取得突破性进展。 目的：观察肌腱损伤局部植入链霉素复合降解膜后腱周结缔组织的增生。 方法：切断SD大鼠跟腱后,随机数字表法分为3组,分别在肌腱断端缝合处植入链霉素复合缓释降解膜、维生素C复合缓释降解膜、空白缓释降解膜。术后90 d行肌腱损伤局部组织学观察、羟脯氨酸含量和生物力学指标检测。 结果与结论：链霉素复合缓释降解膜组肌腱缝合处内部的成纤维细胞、胶原纤维均较其腱周围、维生素C复合缓释降解膜组、空白缓释降解膜组多;腱缝合处肌腱周围多为正常结构的疏松结缔组织,很少有增生的结缔组织长入肌腱内部;肌腱与周围组织分界清晰,最大抗拉强度、羟脯氨酸含量明显优于其他两组。表明链霉素复合缓释降解膜通过抑制腱周结缔组织增生,防止腱周结缔组织增生长入腱内,从而减轻或防止粘连形成。

Connective tissue changes at the position of tendon injury following implantation of streptomycin combined with slow-releasing biodegradable membrane

BACKGROUND:Domestic and foreign scholars have used biological, non-biological, drug and other methods, such as biofilm, sodium hyaluronate, cellulose sealant, to perform a large number of studies on anti-adhesion tendon repair. However, there is no breakthrough up to now. OBJECTIVE:To observe the proliferation of connective tissues after implantation of streptomycin combined with slow-releasing biodegradable membrane. METHODS:After tendon cutting, SD rats were randomly divided into three groups: In group A, streptomycin combined with slow-releasing biodegradable membrane was implanted into the sutured site of Achilles tendon; in group B, vitamin C combined with slow-releasing degradable membrane were implanted; in group C, single slow-releasing degradable membrane was used. After 90 days, histological observation was performed for tendon injury as well as tractus hydroxyproline content and biomechanics indexes were measured. RESULTS AND CONCLUSION: In the group A, there were more fibroblasts and collagen fibers in the sutured site of Achilles tendon than its surrounding tissues, group B and group C. Surrounding the sutured site, many loose connective tissue with normal structure could be seen, but few hyperplastic connective tissues grew into the tendon. A clear demarcation existed between the tendon and its peripheral tissues. The content of hydroxyproline and the ultimate tensile strength in group A were higher than those of groups B and C. The findings show that streptomycin combined with slow-releasing biodegradable membrane can alleviate or prevent tendon adhesion through inhibiting peripheral connective tissue proliferation and preventing connective tissue to grow into the tendon.