脊髓全横断大鼠模型的构建 |
| |
作者姓名: | 孟步亮 巴迎春 宋士娜 陈姗姗 李力燕 王廷华 |
| |
作者单位: | 昆明医学院,解剖学教研室,神经科学研究所,云南省昆明市 650031 |
| |
摘 要: | 背景:脊髓全横断模型在造模时常难以保证神经纤维的完全离断。
目的:构建大鼠脊髓全横断损伤模型。
方法:将大鼠随机分为模型组和假手术组。模型组构建脊髓T10节段全横断模型;假手术组动物仅打开椎管与硬脊膜而后缝合,但不损伤脊髓。建模后1,3,5,7 d分别进行BBB评分以评估后肢运动功能,检测其体感诱发电位和运动诱发电位来评估神经传导通路的完整性,并行形态学观察来评估脊髓肉眼观病理形态。
结果与结论:与假手术组相比,模型组大鼠在建模后1,3,5,7 d时,其BBB评分降低(P < 0.01),未检测出体感和运动诱发电位。形态学观察结果显示模型组大鼠脊髓完全横断,而假手术组脊髓形态完整。结果提示实验成功构建了大鼠脊髓全横断模型。
|
关 键 词: | 脊髓损伤 体感诱发电位 运动诱发电位 动物模型 组织构建 |
收稿时间: | 2010-09-02 |
Establishment of spinal cord transection injury models in rats |
| |
Authors: | Meng Bu-liang Ba Ying-chun Song Shi-na Chen Shan-shan Li Li-yan Wang Ting-hua |
| |
Institution: | Department of Anatomy,Institute of Neuroscience, Kunming Medical College, Kunming 650031, Yunnan Province, China |
| |
Abstract: | BACKGROUND:The spinal cord injury (SCI) models reported in literature were short of determinacy and reliability.
OBJECTIVE:To establish an accurate and reliable rat spinal cord transection injury models.
METHODS:Rats were divided into model and sham-surgery groups at random. Model group prepared for T10 transection injury models; vertebral canal and spinal dura mater were exposed and sutured in the sham-surgery group. The motor function of hind limbs was evaluated by Basso, Beattie and Bresnahan (BBB) scores; the integrality of the nervous pathways was detected by somato-sensory evoked potential (SEP) and motor evoked potential (MEP), in addition, the pathologic and gross pathology of spinal cord were performed at 1, 3, 5 and 7 days after modeling.
RESULTS AND CONCLUSION:Compared with the sham-surgery group, the BBB scores of the model group were decreased at 1, 3, 5 and 7 days after modeling (P < 0.01). The SEP and MEP were not evoked out in the model group. The spinal cords were fully transected in the model group, which was intact in the sham-surgery group. that of. The result suggested that a spinal cord transection injury model is established successfully. |
| |
Keywords: | |
|
| 点击此处可从《》浏览原始摘要信息 |
| 点击此处可从《》下载免费的PDF全文 |
|