大鼠骨髓间充质干细胞成骨分化过程中Hedgehog信号分子的表达

背景：Hedgehog信号通路是一个在胚胎阶段调控多种组织器官发育的重要信号通路,在成骨发育方面具有重要的作用。但Hedgehog信号分子在大鼠骨髓间充质干细胞体外诱导分化为成骨细胞过程中的作用尚未清楚。 目的：体外诱导大鼠骨髓间充质干细胞向成骨分化,检测Hedgehog信号分子在成骨诱导分化过程中的变化。 方法：从大鼠骨髓中分离得到骨髓间充质干细胞,进行地塞米松成骨诱导,通过免疫组化方法鉴定成骨的情况,Western Blot方法检测 Hedgehog信号分子SHH和IHH在骨髓间充质干细胞成骨分化过程中的表达。 结果与结论：成功分离得到骨髓间充质干细胞,地塞米松诱导培养7,14,21 d 后,Ⅰ型胶原的表达量逐渐增加;在诱导成骨分化过程中,SHH蛋白表达升高,诱导组的表达明显高于未诱导组的表达(P < 0.05),而IHH蛋白的表达降低,诱导组的表达明显低于未诱导组的表达(P < 0.05)。结果提示,Hedgehog信号分子参与地塞米松诱导骨髓间充质干细胞分化为成骨细胞的过程,且SHH和IHH在间充质干细胞诱导成骨过程中的作用有差异。

Expression of Hedgehog signaling molecules in the osteogenesis of rat bone marrow mesenchymal stem cells

BACKGROUND:Hedgehog signaling pathway is an important signal pathway, which regulates the development of variety of tissues and organs in the embryonic stage. It also plays an important role in osteogenesis. However, there are few data about the role of Hedgehog signaling molecules in osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) in vitro. OBJECTIVE:To investigate the expression of Hedgehog signaling molecules during osteogenic differentiation in rat BMSCs in vitro. METHODS:BMSCs were derived from rat bone marrow, cultured and induced by dexamethasone. Identification of osteoblasts was performed by immunohistochemistry. Level of Sonic hedgehog (SHH) and Indian hedgehog (IHH) during osteogenic differentiation of BMSCs were detected by Western Blot. RESULTS AND CONCLUSION:BMSCs were successfully obtained. Expression of collagen type I was increased on days 7, 14 and 21 following dexamethasone induction. In the osteogenic differentiation, a higher expression of SHH protein was detected in osteoblast induced group compared with non-induced group at the same time point (P < 0.05). While IHH expression was decreased and significantly lower in osteoblast induced group compared with non-induced group (P < 0.05). The results indicate that Hedgehog signaling molecules involved in the osteogenic differentiation of BMSCs induced by dexamethasone, and SHH and IHH had difference in osteogenic differentiation of BMSCs.