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长波紫外线照射皮肤成纤维细胞表皮生长因子受体的表达
引用本文:刘 垠,刘 流,王继华,鲁开化,伍尚敏,杨瑞年,龙庭凤,袁瑞宏. 长波紫外线照射皮肤成纤维细胞表皮生长因子受体的表达[J]. 中国组织工程研究, 2011, 15(11): 1965-1968. DOI: 10.3969/j.issn.1673-8225.2011.11.016
作者姓名:刘 垠  刘 流  王继华  鲁开化  伍尚敏  杨瑞年  龙庭凤  袁瑞宏
作者单位:1昆明医学院第一附属医院整形外科,云南省昆明市6500322昆明医学院第二附属医院整形外科,云南省昆明市6501013解放军第四军医大学第一附属医院整形外科,陕西省西安市 7100324昆明市第一人民医院整形外科,云南省昆明市650011
基金项目:云南省自然科学基金面上项目(2004C0047M)。
摘    要:背景:长波紫外线与人体皮肤老化关系密切,主要作用于表皮和真皮全层。目的:观察长波紫外线照射对体外培养的皮肤成纤维细胞的表皮生长因子受体表达的影响。方法:体外培养人成纤维细胞,将细胞分为对照组、长波紫外线照射5,10,20 J/cm2组。照射24 h后,分别用实时PCR和Western blot检测表皮生长因子受体mRNA和蛋白的表达情况。结果与结论:体外培养的皮肤成纤维细胞随着长波紫外线照射剂量的增加,表皮生长因子受体的mRNA与蛋白水平均显著增加,提示长波紫外线可以诱导成纤维细胞里的表皮生长因子的表达。

关 键 词:长波紫外线  光老化  表皮生长因子受体  成纤维细胞  皮肤  
收稿时间:2011-01-04

Influence of ultraviolet light A on epidermal growth factor receptor expression in fibroblasts in vitro
Liu Yin,Liu Liu,Wang Ji-hua,Lu Kai-hua,Wu Shang-min,Yang Rui-nian,Long Ting-feng,Yuan Rui-hong. Influence of ultraviolet light A on epidermal growth factor receptor expression in fibroblasts in vitro[J]. Chinese Journal of Tissue Engineering Research, 2011, 15(11): 1965-1968. DOI: 10.3969/j.issn.1673-8225.2011.11.016
Authors:Liu Yin  Liu Liu  Wang Ji-hua  Lu Kai-hua  Wu Shang-min  Yang Rui-nian  Long Ting-feng  Yuan Rui-hong
Abstract:BACKGROUND:Ultraviolet A has a close relationship with skin photoaging, which impact on epidermis and dermis.OBJECTIVE:To explore the influence of ultraviolet A on the epidermal growth factor receptor expression of human skin fibroblasts in vitro.METHODS:Adult’s fibroblasts were divided into the control and ultraviolet A irradiation (5, 10, 20 J/cm2) groups. The epidermal growth factor receptor mRNA and protein were detected by real-time PCR and Western blot. RESULTS AND CONCLUSION:The epidermal growth factor receptor mRNA and protein levels had upgraded as the dose of ultraviolet A irradiation increased. It suggests that ultraviolet A can lead to epidermal growth factor receptor expression in fibroblasts.
Keywords:
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