构建大腿软组织损伤模型兔与跌创散的修复作用

背景：中药大黄、黄柏有活血祛瘀,消肿止痛,治疗软组织损伤的效果。目的：观察以大黄、黄柏为主药的中药对实验性软组织损伤兔血液流变学及损伤软组织前列腺素E2表达的影响。方法：将健康成年新西兰大耳白兔36只随机分为3组：中药组﹑模型组、正常组,前2组采用打击法建立新西兰大耳白兔右腿急性软组织损伤模型。造模30 min,中药组于损伤处敷跌创散,每日1次,模型组及正常组不使用任何药物治疗。于造模后第5天,观察兔损伤软组织前列腺素E2的表达及血液流变学指标的变化;造模后第5,13天,苏木精-伊红染色结合病理评分观察兔损伤软组织的恢复情况。结果与结论：与模型组比较,中药组损伤软组织病理评分更高(P < 0.05),较早接近于正常组织。同时,中药组损伤软组织前列腺素E2的水平和血液黏度较模型组低(P < 0.05)。说明,中药跌创散可能通过降低损伤局部组织炎症递质前列腺素E2的表达、改善血液流变学指标发挥其对软组织损伤的治疗作用。

Construction of rabbit models with thigh soft tissue injury and repair effect of traditional Chinese medicine Diechuangsan

BACKGROUND:Traditional Chinese medicine Rheum palmatum L. and Cortex Phellodendri Chinensis have the property of activating blood circulation and removing stasis, as well as deswelling and alleviating pain, which obtain good outcomes in treating soft tissue injury. OBJECTIVE:To study the effect of Diechuangsan (DCS) on experimental soft tissue injury and prostaglandin E2 expression.METHODS:Totally 36 New Zealand big ear white rabbits were randomly divided into 3 groups, namely, DCS, model and control groups. Rabbits were established acute soft tissue injury models and treated by DCS at 30 minutes after model preparation, once per day. No medication was performed in the model and control groups. Prostaglandin E2 expression and hemorheology index changes were observed at 5 days after model preparation. The recovery of injured soft tissues was observed by hematoxylin-eosin staining and pathological score at 5 and 13 days after model preparation.RESULTS AND CONCLUSION: Compared with the model group, the pathological score of the DCS group was higher (P < 0.05), which was earlier close to the normal tissues. In addition, the prostaglandin E2 expression and blood viscosity of the DCS group was lower than that of the model group (P < 0.05). It is suggested that DCS can repair injured soft tissues via decreasing inflammatory mediators prostaglandin E2 and reducing injury animal blood viscosity.