丝蛋白应用于牙周组织工程的可行性

背景：丝蛋白是有利于表皮细胞、成纤维细胞、成骨细胞、血管内皮细胞、胶质细胞黏附和生长的一种新型生物材料。 目的：评估丝蛋白作为支架材料应用于牙周组织工程的可行性。 方法：采用组织块法培养人牙周膜细胞，将第5代细胞悬液以2×10⁷ L^-1的浓度接种到丝蛋白支架材料上复合培养，并以1%，10%，50%，100%的丝蛋白支架浸提液培养，观察人牙周膜细胞在丝蛋白上及在丝蛋白浸提液中生长状况，用MTT法测定浸提液培养人牙周膜细胞的活力。 结果与结论：扫描电镜可见人牙周膜细胞在丝蛋白支架上伸展充分，生长旺盛，不同浓度丝蛋白支架浸提液培养对人牙周膜细胞的增殖与碱性磷酸酶活性均无影响。说明丝蛋白材料具有良好的生物相容性、独特的力学性能，可作为人牙周膜细胞黏附生长的理想支架材料较好地应用于牙周组织工程中。

Feasibility of silk fibroin proteins used in periodontal tissue engineering

BACKGROUND:Silk fibroin protein is a new type of biomaterial that is beneficial to the adhesion and growth of skin cells, fibroblasts, osteoblasts, endothelial cells, and glial cells. OBJECTIVE:To evaluate the feasibility of the silk fibroin protein as an ideal scaffold material for periodontal tissue engineering. METHODS:Silk fibroin protein composite scaffolds were prepared through a freeze-drying method. The periodontal ligament cells (2×10⁷/L) were collected and seeded on three-dimensional framework of silk fibroin protein (1%, 10%, 50%, 100%). The cell growth and the influences of the silk fibroin protein extracts on cell proliferation and differentiation were observed.  RESULTS AND CONCLUSION: The periodontal ligament cells adhered and proliferated on the three-dimensional framework of silk fibroin protein, the different concentrations of silk fibroin protein extracts had no influences on the cell proliferation and differentiation. Silk fibroin protein can be used as an ideal scaffold material for cell adhesion and growth in periodontal tissue engineering.