聚己内酯/丝胶蛋白静电混纺膜炎症响应的体内评价

背景：研究表明，丝胶蛋白可以增加哺乳动物细胞的增殖，促进伤口愈合，具有良好的抗菌、抗氧化、抗癌的活性，同时具有良好的生物相容性和可降解性，是一种理想的组织工程材料。然而目前对丝胶蛋白能否引起炎症响应仍存在争论。目的：评价聚己内酯/丝胶蛋白静电混纺膜的炎症响应。方法：将不同混合比例的聚己内酯/丝胶蛋白纳米纤维薄膜体内埋植入大鼠背部肌肉，4周后取出进行组织学观察，通过苏木精-伊红染色和巨噬细胞抗体CD68免疫组织化学染色评价其炎症响应。结果与结论：聚己内酯/丝胶蛋白7∶3组混纺膜炎症反应与丝素蛋白膜类似，小于聚己内酯/丝胶蛋白6∶4组、聚己内酯/丝胶蛋白5∶5组和聚己内酯组；聚己内酯/丝胶蛋白6∶4组与聚己内酯/丝胶蛋白5∶5组与聚己内酯组类似。提示聚己内酯/丝胶蛋白静电混纺膜不会引起显著的炎症响应，少量添加丝胶蛋白可以降低聚己内酯的炎症反应，随着丝胶蛋白含量的增加，炎症反应呈上升趋势。

Inflammatory response evaluation for electrospun polycaprolactone/silk sericin membranes in vivo

BACKGROUND:Studies have shown that silk sericin (SS) can accelerate the proliferation of mammalian cells and promote wound healing. SS has good activities for antibacteria, antioxidation and anticancer. SS also has a good biocompatibility and biodegradability. It is an ideal material for tissue engineering, however, there is still a controversy of whether SS causes inflammatory response so far.OBJECTIVE:To evaluate the inflammatory response of electrospun polycaprolactone (PCL)/SS membranes.METODS:Materials with different PCL/SS ratios were implanted into the back muscles of rats and were taken out after 4 weeks. The inflammatory response was evaluated by hematoxylin eosin staining and macrophage antibody CD68 immunohistochemistry. RESULTS AND CONCLUSION: The inflammatory response of the 7:3 group of PCL/SS membranes is similar to that of silk fibroin membrane and was less than those of the 6:4, 5:5 and PCL groups. The 6:4 and 5:5 groups have similar inflammatory response with PCL group. Electrospun PCL/SS membranes can not cause significant inflammatory response. A little amount of SS can decrease the inflammatory response of PCL membrane. However, the inflammatory response of PCL/SS membrane can increase as the ratio of SS to PCL increased.