结直肠癌组织中CD44+肿瘤细胞与S期标志物增殖细胞核抗原的关系

背景：目前尚未找到结直肠癌干细胞理想的标志物,结合周期蛋白能更精确的找到GO/G1期癌干细胞。 目的：了解结直肠癌组织中CD44和增殖细胞核抗原PCNA的表达及CD44⁺肿瘤细胞与S期标志物增殖细胞核抗原PCNA的关系。 方法：采用组织芯片检测、免疫组织化学和双重免疫组织化学染色技术检测61例结直肠癌组织、10例正常肠黏膜组织中增殖细胞核抗原PCNA和CD44表达情况。 结果与结论：在结直肠癌组织中,PCNA⁺细胞数远多于CD44⁺细胞数,CD44⁺癌细胞形态有两种,一种核较大,染色质边集,呈空泡状,核膜核仁清晰,这些细胞大多数呈PCNA阳性;另一种核较小,染色质致密,呈小圆形,部分表达PCNA。在大多数病例中,CD44⁺细胞中90%以上的瘤细胞同时为PCNA阳性,只有(3.38±2.08)%细胞为单纯PCNA阳性。说明结直肠癌组织中大多数CD44⁺细胞呈现S期标志物PCNA阳性,处于S期;只有极少数CD44⁺/PCNA-处于G0/G1期的细胞可能为肿瘤干细胞。

Relationship of CD44+ tumor cells and S-phase marker proliferating cell nuclear antigen in colorectal carcinoma

BACKGROUND:An ideal surface marker of cancer stem cells has not been found in colorectal carcinoma and it is very useful to find cancer stem cells in G0/G1 phase accurately by detecting the cell cycle.  OBJECTIVE:To investigate the expression of CD44 and proliferating cell nuclear antigen (PCNA) and the relationship between CD44⁺ tumor cells and S-phase marker PCNA in colorectal carcinoma. METHODS:The expression of CD44 and PCNA were detected by tissue chip, immunohistochemical staining and double immunohistochemical staining methods in 61 cases of colorectal carcinoma and 10 cases of normal mucosa. RESULTS AND CONCLUSION:In colorectal carcinoma, the number of PCNA⁺ tumor cells was remarkably more than that of CD44⁺ cells. There were two kinds of morphous of CD44⁺ tumor cells: one presented with bigger vacuolus nucleus, chromatin margination, clear nuclear membrane and nucleolus, and most of these tumor cells expressed PCNA; Another showed smaller nucleus, chromatin dense, and partly expressed PCNA. In most cases, more than 90% of CD44⁺ tumor cells were PCNA⁺, but just (3.38±2.08)% showed PCNA^-. In colorectal carcinoma, most of CD44⁺ tumor cells expressed PCNA and stayed in S-phase. Just few CD44⁺/PCNA^- staying in G0/G1 phase may be cancer stem cells.