数字化载银羟基磷灰石人工骨抗菌性及生物相容性

背景：载银珊瑚羟基磷灰石作为一种新型抗菌植骨材料，受到越来越多的关注，作为植入物需与人体具有良好的生物相容性。目的：观察数字化载银珊瑚羟基磷灰石人工骨材料的抗菌性及生物相容性。方法：将珊瑚羟基磷灰石粉末浸泡于不同浓度的硝酸银溶液，制备出不同含银量的载银羟基磷灰石，再将其与聚乳酸混合，并通过选择性激光烧结快速成形制备出具有特殊形状的数字化载银抗菌人工骨材料。结果与结论：连续光源原子吸收光谱仪测定珊瑚羟基磷灰石浸泡于10^-2，10^-3，10^-4，10^-5 mol/L AgNO₃中制备的载银人工骨中Ag+的含量分别为2.31×10^-1%，3.18×10^-2%，6.75×10^-3%，6.05×10^-4%。体外抑菌圈实验表明浸泡10^-2 mol/L AgNO₃中制备的载银人工骨材料对金黄色葡萄球菌和大肠杆菌的抑菌圈直径分别为(13.00±1.52)mm 及(12.30±1.65)mm；浸泡10^-3 mol/L AgNO₃中制备的载银人工骨材料分别为(11.50±0.73) mm及(11.00±0.46) mm。浸泡10^-4，10^-5 mol/L AgNO₃载银人工骨材料对两种细菌均无抑菌圈产生。细胞毒性试验结果表明100%浸泡10^-2，10^-3，10^-4，10^-5 mol/L AgNO₃的载银珊瑚羟基磷灰石人工骨材料的细胞毒性分别为3级、1级、0级、0级。急性全身毒性试验表明浸泡10^-3 mol/L AgNO₃中的人工骨浸提液对小鼠无明显的急性毒性反应，具有良好的安全性。结果表明浸泡10^-3 mol/L AgNO₃中的载银珊瑚羟基磷灰石人工骨在体外对金黄色葡萄球菌及大肠杆菌有明显抗菌作用，且具有良好的生物相容性及安全性。

Antibacterial property and biocompatibility of digital silver loaded hydroxyapatite as artificial bone

BACKGROUND:The silver loaded coral hydroxyapatite (CHA-Ag) has received increasing attention as a new type of antibacterial bone graft material. Implant materials need well biocompatibility with human body.OBJECTIVE:To observe the antibacterial property and biocompatibility of digital CHA-Ag as artificial bone.METHODS:The coral hydroxyapatite powder was immersed in silver nitrate solution (AgNO₃) with different concentrations in order to prepare CHA-Ag containing different contents of Ag⁺. Then the prepared CHA-Ag was mixed with poly lactic acid and burned by selective laser to prepare digital artificial bone material containing silver with special shape rapidly.RESULTS AND CONCLUSION: The continuum source atomic absorption spectrometer showed that the Ag⁺ contents of CHA-Ag prepared with 10^-2, 10^-3, 10^-4 and 10^-5 mol/L AgNO₃ were 2.31×10^-1%, 3.18×10^-2%, 6.75×10^-3%, and 6.05×10^-4% respectively. The inhibition ring test demonstrated that the inhibition ring diameter of CHA-Ag by 10^-2 mol/L AgNO₃ against staphylococcus aureus and Escherichia coli were (13.00±1.52) mm and (12.30 ±1.65) mm respectively; while that of 10^-3 mol/L were (11.50±0.73) mm and (11.00±0.4) mm respectively, and no inhibition ring occurred in that of 10^-4 and 10^-5 mol/L AgNO₃. Cytotoxicity test manifested that the cytotoxicity grades were 3, 1, 0 and 0 respectively according to the CHA-Ag prepared with 10^-2, 10^-3, 10^-4 and 10^-5 mol/L AgNO₃. General toxicity test indicated that the artificial bone leaching liquor with 10^-3 mol/L AgNO₃ had no obvious acute cytotoxicity in mice. The CHA-Ag prepared with 10^-3 mol/L AgNO₃ has good biocompatibility and biological security, can inhibit the growth of staphylococcus aureus and Escherichia coli in vitro.