地塞米松联合脂多糖诱导股骨头坏死模型的构建

背景：对于研究股骨头坏死的发病机制及治疗方法，建立与股骨头坏死病程相似的动物模型是非常重要的。 目的：观察地塞米松联合脂多糖诱导兔股骨头坏死的实验效果。 方法：健康雄性新西兰大白兔30只，随机分为模型组(n=20)和对照组(n=10)。模型组经耳缘静脉注射2次10 μg/kg的脂多糖，再肌肉注射3次地塞米松25 mg/kg，对照组注射同等剂量的生理盐水。 结果与结论：4周后，CT扫描显示模型组兔股骨头不同程度的骨密度不均匀。显微CT观察发现，模型组骨体积分数、骨矿物质密度、骨矿物质含量和骨小梁厚度均显著低于对照组，而骨小梁间隙高于对照组(P < 0.05)。组织学切片见模型组骨细胞陷窝空疏，脂肪细胞增多，部分血管栓塞，其中存活动物的骨坏死率和骨陷窝空虚率均明显低于对照组。说明地塞米松联合脂多糖可有效建立骨坏死模型。

Constructing a rabbit model of necrosis of the femoral head by the combination of dexamethasone and lipopolysaccharide

BACKGROUND:An ideal animal model of necrosis of the femoral head is beneficial to the studies on the cause of disease, pathogenesis and treatment. OBJECTIVE:To research the experimental result of necrosis of rabbit femoral head induced by the combination of dexamethasone and lipopolysaccharide. METHODS:Thirty male New Zealand rabbits were divided into experimental group (20 rabbits) and control group (10 rabbits) randomly. Two injections of 10 μg/kg body lipopolysaccharide and three injections of 25 mg/kg dexamethasone were performed in the experimental group. Physiologic saline was injected in the control group. RESULTS AND CONCLUSION:The CT scanning showed non-uniform of bone density of the femoral head in the experimental group. The indexes of Micro-CT bone metrological parameters were significantly lower in the experimental group than in the control group (P < 0.05). Bone cell lacunae, increased fat cells and thrombokinesis were observed in histopathological examination. The osteonecrosis rate of survived rabbits as well as the rate of empty bone lacunae was significantly lower in the experimental group than the control group. It is indicated that the combination of dexamethasone and lipopolysaccharide can induce an osteonecrosis model.