点种法移植软骨细胞修复关节软骨缺损

背景：生物性的重建虽能达到修复缺损,重建关节面的目的,但功能上难以与正常软骨一致。应用可降解的聚合物把移植的软骨细胞包埋起来进行移植,可能获得真正意义上的透明软骨。 目的：观察以同种异体兔软骨细胞胶原包埋后,点种法移植软骨细胞修复关节软骨缺损的效果。 方法：新西兰纯种兔制备膝关节全层软骨缺损后分为3组：分别进行胶原包埋软骨细胞点种法移植、单纯软骨细胞点种法移植和仅在大面积软骨缺损的软骨下钻孔。 结果与结论：术后2,4,12,24周观察组织学动态变化,发现胶原包埋软骨细胞点种法移植组能获得透明软骨修复,而软骨细胞点种法组和单纯软骨下骨钻孔组缺损区仅为纤维组织填充,并且胶原包埋软骨细胞点种法移植组兔各期平均组织学和组织化学得分均高于其他两组(P < 0.01)。说明胶原包埋点种法软骨细胞移植能获得透明软骨修复,尤其适用于大面积软骨缺损。

Point implantation of allograft chondrocytes for repairing articular cartilage defects

BACKGROUND:Although many biological reconstruction can repair defects and rebuild articular surfaces, the function is inferior to normal cartilage. Transplantation of biodegradable polymer-coated cartilage cells may obtain real hyaline cartilage. OBJECTIVE:To observe effects of collagen-embedded chondrocytes by point implantation method on repairing articular cartilage defects. METHODS:Articular cartilage defects were made in the knee of New Zealand rabbits. The rabbits were randomly divided into three groups, treated with collagen gel-embedded chondrocytes, chondrocytes or no treatment. RESULTS AND CONCLUSION:Histological changes were observed at 2, 4, 12 and 24 weeks postoperatively. Hyaline cartilage was observed in rabbits following transplantation of collagen gel-embedded chondrocytes, while only fibrous tissues were observed in the defects in the other two groups. Moreover, mean histological and histochemical scores were greater in the rabbits treated with collagen gel-embedded chondrocytes compared with the other groups (P < 0.01). Results show that transplantation of collagen gel-embedded chondrocytes can obtain hyaline cartilage, and can be used for repairing large-area cartilage defects.