姜黄素对瘢痕疙瘩成纤维细胞周期及其核转录因子κB信号转导通路的影响

背景：有研究表明姜黄素具有抗器官纤维化的作用,具抑制增殖及诱导G0/G1期细胞累积的作用,但姜黄素可否影响瘢痕疙瘩成纤维细胞的细胞周期,活化核转录因子κB通路,从而抑制瘢痕增生至今少有报道。目的：观察姜黄素对体外培养人瘢痕疙瘩成纤维细胞周期的影响及其核转录因子κ B信号转导通路的变化。方法：瘢痕疙瘩成纤维细胞进行体外原代培养,待细胞融合后传代,取第6~8代对数生长期的成纤维细胞用于实验。将不同浓度姜黄素(10,20,30,40,50,60 µmol/L)分别对瘢痕疙瘩成纤维细胞干预。用流式细胞仪测定细胞周期;免疫组织化学法检测核转录因子κ B的表达。结果与结论：姜黄素呈剂量和时间依赖性抑制成纤维细胞的增生(P < 0.05),使细胞周期停滞在G1期,核转录因子κB表达随姜黄素剂量的增大而减小(P < 0.05)。结果显示,姜黄素能抑制瘢痕疙瘩成纤维细胞的增殖,并使细胞周期停滞在G1期,姜黄素可能通过抑制核转录因子κB信号转导通路的活化,从而发挥其抗纤维化的作用。

Effect of curcumin on keloid fibroblasts cycle and nuclear factor-kappa B signaling pathways

BACKGROUND:Studies have indicated that curcumin can inhibit keloid fibroblasts. It can suppress keloid fibroblasts (KFB) proliferation and induce cell cycle arrest at G0/G1 phase. However, whether curcumin can inhibit KFB proliferation remains poorly understood.                                             OBJECTIVE:To study the effect of curcumin on cultured human KFB proliferation and its signal transduction pathway. METHODS:Keloid fibroblasts were primary cultured in vitro, passaged after cell fusion, and 6-8 passages of cells with logarithmic growth were used. Different concentrations of curcumin (10, 20, 30, 40, 50, 60 μmol/L) were used to intervene keloid fibroblasts. Cell cycle was determined by flow cytometry, and expression of nuclear factor-κB was detected by immunohistochemistry.RESULTS AND CONCLUSION:Curcumin inhibited KFB hyperplasia in a dose- and time-dependent manner (P < 0.05), and arrested cell cycle at G1 phase, nuclear factor-κB expression increased with the dose of curcumin decreased (P < 0.05). Curcumin can inhibit KFB proliferation and arrest cell cycle in G1 phase. Curcumin might inhibit nuclear factor-κB signal transduction pathway activation, and thus play its anti-fiber role.