肝动脉注射锰锌铁氧体磁性纳米颗粒治疗兔VX2肝癌

背景：与传统的热疗方法相比，磁流体热疗具有很好的磁响应性，在一定高频交变磁场下能实现肿瘤热疗的自动控温和靶向治疗等优点。目的：制备锰锌铁氧体磁性纳米颗粒，观察其介入治疗兔VX2 肝癌的效果。方法：采用开腹后瘤粒悬浮液针头注入法制作兔VX2 肝癌模型，造模后14 d随机数字表法分为对照组(生理盐水)、锰锌铁氧体磁性纳米颗粒非热疗组、锰锌铁氧体磁性纳米颗粒热疗组、阿霉素组，均采用3F导管从右侧股动脉选择至肝固有动脉动脉注入药物后拔管。锰锌铁氧体磁性纳米颗粒热疗组于介入后行热疗3次。介入治疗后14 d取肝脏组织测量肿瘤大小，并做病理组织学检查。结果与结论：透射电镜下观察制备的锰锌铁氧体磁性纳米颗粒为球形，大小为20~30 nm，在交变磁场下有良好的磁感应升温能力。治疗后14 d，锰锌铁氧体磁性纳米颗粒热疗组肿瘤大面积坏死，肿瘤抑制率达到70.84%，明显高于锰锌铁氧体磁性纳米颗粒非热疗组、阿霉素组与对照组(P < 0.05或P < 0.01)。说明锰锌铁氧体磁性纳米颗粒可吸收电磁波转化为热能，通过介入治疗可显著抑制兔VX2 肝癌生长。

Hepatic artery injected with Mn-Zn ferrite magnetic nanoparticles for treatment of rabbit VX2 liver tumor

BACKGROUND:Compared with traditional thermotherapy method, magnetic fluid hyperthermia (MFH) has good magnetic response, and which can achieve automatic temperature control and targeting of tumor thermotherapy in certain high frequency alternating magnetic field.OBJECTIVE:To prepare Mn-Zn ferrite (MZF) magnetic nanoparticles, and to observe the inhibition effects of MZF magnetic nanoparticles combined with hepatic artery interventional therapy on rabbit VX2 liver tumor. METHODS:Rabbit VX2 liver tumor models were established by implanting tumor piece and cell suspension via abdominal incision. At 14 days after modeling, a total 24 rabbits were randomly divided into 4 groups as follows: control group (sodium chloride), MZF group, MZF hyperthermia group, adriamycin group, each groups contain 6 VX2 rabbits. The rabbit hepatic proper artery was selectively catheterized by 3F micro-catheters via right femoral artery. The rabbits of MZF hyperthermia group were exposed to alternating magnetic field for three times. At 14 days after interventional operation, the tumor size of liver tissue was detected and harvested for histopathology examination. RESULTS AND CONCLUSION:MZF nanoparticles were approximately global and their average diameter was 20-30 nm examined by transmission electron microscope (TEM), and they had good magnetic responsiveness under alternating magnetic field. At 14 days after treatment, large areal necrosis arose in tumor in MZF hyperthermia group, the tumor-inhibiting rate was 70.84% higher than the other groups, and the difference was significant (P < 0.05 or P < 0.01). MZF nanoparticles can absorb electromagnetic waves into heat, through interventional therapy which can significantly inhibit the growth of rabbit VX2 liver tumor.