益胃冲剂对胃癌前病变大鼠粘膜上皮细胞DNA量及AgNOR数的影响

目的：从实验角度对益胃冲剂的药效进行观察，并对其逆转机制加以探讨。方法：50只SD大鼠随机分为正常组，模型组，益胃冲剂高，低剂量治疗组，维酶素对照组，分别对各组病理变化，胃粘膜上皮细胞AgNOR计数，DNA相对含量及DNA相对倍体值进行观察。结果：模型组大鼠87．5％出现粘膜变薄，腺体萎缩，100％出现炎细胞浸润，66．5％出现肠上皮化生，37．5％出现异型增生，其粘膜上皮细胞AgNOR计数，DNA相对含量及DNA相对倍体值明显增高，而治疗组粘膜变薄，腺体萎缩，炎细胞侵润，肠上皮化生，不典型增生的发生率分别为高剂量组：11．1％，11．1％，11．1％，0，0和低剂量组：22．5％，11．1％，22．2％，11．1％，0较模型组的87．5％，87．5％，100％，62．5％，37．5％明显改善（P＜0．01），AgNOR计数、DNA相对含量及相对倍体值较模型组明显降低，与维酶素对照组丰厚显差异（P＜0．05）。结论：益胃冲剂能有效地逆转胃粘膜癌前病变，其逆转机制可能与其降低病变细胞DNA相对含量，抑制其异常活跃的增生状态有关。

Effects of YiWeiChongJi on DNA content and AgNOR counting in epithelial cells of gastric mucosa in rats with precancerous lesion of gastric carcinoma

AIM: To study the curative effects and the mechanism of YiWeiChongJi. METHODS: 50 rats were divided randomly and evenly into 5 groups: Normal group, Model group, Therapy group with high dosage of YiWeiChongJi, Therapy group with low dosage of YiWeiChongJi and Control group with WeiMeiSu. The pathological changes, AgNOR counting, DNA relative content and U levels of epithelial cells of gastric mucosa in each group were examined. RESULTS: In Model group, mucosa got thin and gland got atrophied in 87.5% of the rats, infiltration of inflammation cells presented in 100%. Intestinal metaplasia was seen in 66.5% of the rats and atypical hyperplasia in 37.5%. The AgNOR counting, DNA content and U level of epithelial cells increased markedly. However, in the Therapy group with low or high dosage of YiWeiChongJi, pathological changes ameliorated significantly ( P <0.01). Compared with those of the Model group, the AgNOR counting and DNA content, U level of epithelial cells declined markedly, which were statistically different from those of the Control group ( P <0.05). CONCLUSION: YiWeiChongJi can effectively reverse the precancerous lesions in gastric carcinoma and its mechanism of reversion probably relates to the reduction of DNA content and restraint of abnormal active hyperplasia.