早孕妇女外周血和蜕膜组织中自然杀伤细胞亚群的变化

目的和方法：用流式细胞仪检测正常非孕及早孕妇女外周血和蜕膜自然杀伤细胞表面标记ＣＤ５６和ＣＤ１６分子，探讨早孕外周血与蜕之间自然杀伤细胞亚群的差异以及雌、孕激素对外周血自然杀伤细胞的影响。结果：蜕膜以ＣＤ５６细胞为主要淋巴细胞，早孕外周血则以ＣＤ５６ＣＤ１６和ＣＤ１６为主。孕酮在一定范围内增加，可使外周血自然杀伤细胞各亚群的百分率明显增加；在早孕期、雌、孕激素水平的急剧升高可能使外周血ＣＤ５６细胞

Different expression of NK cellular subsets in decidual tissue and peripheral blood

AIM:To determinate the difference of natural killer (NK) cellular subpopulations between decidual tissue and peripheral blood(PB) in early pregnancy, and to investigate the effect of estrogen and progesterone on NK cell of PB.METHODS:Flow cytometry was used to detect the expression of CD56 and CD16 of NK cellular markers on peripheral blood lymphocytes(PBL) in non-pregnancy and on decidual lymphocytes and PBL in early pregnancy. RESULTS: CD56 NK cells were predominant population of decidual lymphocytes, and both CD56 CD16 and CD16 NK cells made up the majority of PBLs in early pregnancy. The percentage of diverse subpopulations of NK cells was significantly enhanced in peripheral blood as progesterone was increased within certain limits; it was possible that CD56 NK cells were likely transformed into CD16 NK cells in peripheral blood when the levels of estrogen and progesterone were sharply raised during early pregnancy. CONCLUSION:The difference of NK cellular subpopulations between peripheral blood and decidual tissues in early pregnancy played an important role in the maintenance of normal pregnancy.