成熟心肌细胞异体移植对心肌梗死后心功能的保护作用

目的:研究异体成熟心肌细胞移植对心肌梗死后心功能的保护作用。方法:成年新西兰兔结扎冠状动脉左前降支建立心肌梗死模型,体外培养异体心肌细胞并用5溴脱氧尿核苷（BrdU）标记,将4×107/ml细胞悬液移植到急性心肌梗死区域。对照组将同量细胞培养液注射到急性心肌梗死区。细胞移植5周后超声心动图测量活体心功能,并进行组织学观察及免疫组化检测。结果:接受细胞移植组心脏功能显著优于对照组,左室重构受到抑制。细胞移植区观察到被标记心肌细胞,同时电镜下观察到心肌细胞之间存在细胞间联系。结论:移植细胞能够在宿主体内存活并与周围组织建立联系抑制左室重构,保护心脏功能。

Adult cardiomyocytes allograft to protect the cardiac function after myocardial infarction

AIM:In order to research the effect of transplantation of homologous adult cardiomyocytes on protecting heart function after myocardial infarction(MI).METHODS:After labeled with BrdU,cultured adult rabbit cardiomyocytes were implanted into the infarction site of rabbits myocardiums via direct injection. The myocardial infarction model was established by ligating the left anterior descending artery. All groups heart function and ventricular remodeling were measured by echocardiography, and the heart slices were processed for histological(H-E)and immunohistological techniques and photographed. RESULTS: In cell-transplanted group heart functions were improved and left ventricular(LV) remoldings were inhibited.Transplanted cells labeled with BrdU were observed by microscope. In addition that implanted cells connected with each other by intercellular junctions. CONCLUSION: Transplanted cells can live in the MI sections and improve heart functions.