血栓通注射液对LPS诱导的兔弥散性血管内凝血的拮抗作用

 目的：研究血栓通注射液对脂多糖（LPS）诱导的兔弥散性血管内凝血（DIC）的影响。方法：对兔耳缘静脉持续滴注LPS以建立兔DIC模型，记录各组动物在24 h后的生存率；全自动血浆分析仪测定丙氨酸氨基转移酶(ALT)和血尿素氮(BUN)；用全自动凝血分析仪检测活化部分凝血活酶时间（APTT）和凝血酶原时间（PT）；用凝固法测定纤维蛋白原含量；全自动血细胞分析仪进行血小板计数；发色底物法测定蛋白C和抗凝血酶Ⅲ的活性；ELISA法检测血浆中肿瘤坏死因子α（TNF-α）的含量。结果：持续从兔耳缘静脉滴注LPS后，DIC模型组动物大量死亡，ALT和BUN显著升高，APTT和PT显著延长，蛋白C和抗凝血酶Ⅲ的活性明显降低，血小板计数明显减少，血浆中TNF-α的含量显著升高。注射血栓通注射液后，动物的死亡率明显下降，ALT和BUN明显下降，APTT和PT明显缩短，血小板计数明显上升，纤维蛋白原含量显著提高，蛋白C和抗凝血酶Ⅲ活性明显提升；血浆TNF-α的含量明显降低。结论：血栓通注射液对LPS所诱导的兔DIC有良好的拮抗作用。

Effect of Xueshuantong injection on LPS-induced DIC in rabbits

AIM：To evaluate the effect of Xueshuantong injection on lipopolysaccharide (LPS)-induced experimental disseminated intravascular coagulation (DIC) in rabbits. METHODS：To establish the LPS-induced DIC model, LPS was continuously injected into the rabbit autricular vein for 6 h. The survival rate of the rabbits was recorded after 24 h. The plasma levels of alanine aminotransferase (ALT) and blood urine nitrogen (BUN) were detected. Activated partial thromboplastin time (APTT), prothrombin time (PT), platelet count and fibrinogen concentration were measured. The activity of protein C, antithrombin Ⅲ (ATⅢ) and the concentration of tumor necrosis factor α (TNF-α) were also determined. RESULTS：The survival rate of DIC rabbits was only 26.7%. BUN and ALT increased obviously. APTT and PT became much longer, platelet count, fibrinogen, protein C and ATⅢ decreased obviously, and plasma TNF-α increased remarkably. The intravenous administration of Xueshuantong injection increased the survival rate of DIC rabbits up to 66.7% in a dose-dependent manner. Xueshuantong injection also decreased the plasma levels of BUN, ALT, APTT, PT and TNF-α significantly, while increased the levels of fibrinogen, platelet, protein C and ATⅢ in plasma. CONCLUSION： Xueshuantong injection has therapeutic effect on LPS-induced DIC in rabbits.