麦冬抑制大鼠心肌成纤维细胞胶原合成的作用及其机制

 目的：观察麦冬抑制大鼠心肌成纤维细胞胶原合成的作用及其机制。方法：以培养的SD大鼠心肌成纤维细胞为观察对象，随机将心肌成纤维细胞分为4组（n=10）：对照组（未用麦冬处理大鼠心肌成纤维细胞）、麦冬（10 μg/L）组、麦冬（20 μg/L）组和麦冬（30 μg/L）组。测定不同组心肌成纤维细胞活力、［3H］-脯氨酸掺入率、TGF-β1 、p-Smad2/3和Smad2/3 蛋白表达的变化。结果：与对照组比较，麦冬（10　μg/L）组大鼠心肌成纤维细胞活力、［3H］-脯氨酸掺入率、TGF-β1 、p-Smad2/3和Smad2/3表达显著降低（P<0.01）。与麦冬（10　μg/L）组比较，麦冬（20 μg/L）组大鼠心肌成纤维细胞活力、［3H］-脯氨酸掺入率、TGF-β1 、p-Smad2/3和Smad2/3表达显著降低（P<0.01）。与麦冬（20 μg/L）组比较，麦冬（30　μg/L）组大鼠心肌成纤维细胞活力、［3H］-脯氨酸掺入率、TGF-β1 、p-Smad2/3和Smad2/3表达显著降低（P<0.01）。结论：麦冬对大鼠心肌纤维化有明显抑制作用，其机制可能与TGF-β1 、p-Smad2/3和Smad2/3 蛋白表达的降低有关。

Inhibitory effect of Ophiopogon japonicus on rat cardiac fibroblasts

AIM：To investigate the inhibitory effect of Ophiopogon japonicus on rat cardiac fibroblast (CFs) and the underlying mechanism. METHODS：Cultured CFs from Sprague-Dawley (SD) rats were randomized into 4 groups: control group (normal rat cardiac fibroblasts), Ophiopogon japonicus of 10 μg/L group, Ophiopogon japonicus of 20 μg/L group and Ophiopogon japonicus of 30 μg/L group. Cell vitality, ［3H］-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3 in CFs were determined. RESULTS：Compared with control, the cell vitality, ［3H］-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3 were significantly decreased in Ophiopogon japonicus of 10 μg/L group. Compared with Ophiopogon japonicus of 10 μg/L group, the cell vitality, ［3H］-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3 were significantly decreased in Ophiopogon japonicus of 20 μg/L group. Compared with Ophiopogon japonicus of 20 μg/L group, the cell vitality,［3H］-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3 were significantly decreased in Ophiopogon japonicus of 30 μg/L group. CONCLUSION：Ophiopogon japonicus may inhibit CFs. These actions are related to the changes of ［3H］-proline incorporation, and the protein expression of TGF-β1, p-Smad2/3 and total Smad2/3.