人肝癌转移抑制基因存在的功能研究

目的 寻求人8号染色体上存在肝癌转移抑制基因的功能证据.方法 前期通过微细胞介导的染色体转移方法(MMCT),将正常人8号染色体导入到大鼠肝癌高转移细胞株C5F中获得的微细胞杂交克隆Ne08/C5F-1～10和大鼠肝癌细胞株CSF,分别被接种到6～7周龄雌性BALB/CA裸鼠皮下进行自发转移实验,观察皮下成瘤及肺部大体转移灶形成情况,肺组织连续切片并计数镜下转移灶,结果进行单因素方差分析.结果 所有微细胞杂交克隆均能皮下成瘤,微细胞杂交克隆的自发转移实验发现有6个杂交克隆出现肺转移表型的明显抑制(P<0.05),其中2个杂交克隆肺转移表型发生完全抑制.结论 人8号染色体对大鼠肝癌高转移细胞株CSF的成功导入改变了CSF的转移表型,提供了人8号染色体上存在肝癌转移抑制基因的直接的功能证据.

The functional evidence for metastasis suppressor genes of liver cancer harboring on chromosome 8

Objective To find out the functional evidence for metastasis suppressor gene in liver cancer on human chromosome 8p. Methods The microcell hybrid clones Neo8/C5F-1-10, which were generated by introducing human chromosome 8 randomly marked with neo gene into C5F cell line by mi-crocell mediated chromosome transfer (MMCT) and the C5F cell line were subcutaneously implanted in 6-7 week-old female BALB/CA nude mice to perform the spontaneous metastasis assay. Serial dissections of the lung tissues were done to count the microscopic metastatic foci. The number of the metastatic foci was compared by one-way ANOVA. Results All the microceli hybrids implanted had grown into measurable tumors subcutaneously. The spontaneous metastasis assay for these microcell hybrid clones showed that 6 clones displayed prominent lung metastasis suppression (P<0.05). Among 2 of them, lung metastases had been totally suppressed. Conclusion The successful introduction of human chromosome 8 into meta-static rat liver cancer cell line had changed the metastatic behavior of C5F, which provided the direct func-tional evidence for the metastasis suppressor gene in hver cancer harboring on human chromosome 8.