生长激素对脑出血大鼠血清白蛋白含量和小肠黏膜形态学的影响

目的 评价重组人生长激素(recombinant human growth hormom,rhGH)对脑出血大鼠血清白蛋白水平和小肠黏膜形态学的影响.方法 采用自体血注射法制作大鼠脑出血模型.56只SD大鼠分为假手术组(n=8),rhGH组(n=24;腹腔注射rhGH,1 U/kg,1次/d)和生理盐水对照组(n=24;腹腔注射等量生理盐水,1次/d),后两组均分别再分为术后1、7和14 d组(每组n=8).检测各组大鼠不同时问点血清白蛋白浓度,HE染色和图像分析观察小肠黏膜形态学改变.结果 脑出血各时间点,生理盐水对照组血清白蛋白水平均较假手术组显著降低(P均<0.01);rhGH组血清白蛋白水平随治疗进程逐渐增高,但仅在14 d时显著高于生理盐水对照组(39.93±1.98)g,L对(37.93±1.57)g,L,P<0.01].脑出血后1 d和7 d时,rhGH组小肠绒毛高度和黏膜厚度与生理盐水对照组无显著差异,但14 d时显著增高(P<0.01).脑出血后1、7和14 d小肠绒毛面积进行性缩小,且rhGH组较生理盐水对照组随治疗进程进行性增高(P<0.01).脑出血后1 d和7 d.rhGH组肠腺深度较生理盐水对照组增高(P<0.01),但14 d时无显著差异;脑出血1 d,rhGH组组肠腺密度较生理盐水对照组显著增高(P<0.01),7 d时增高不显著,14 d时不仅不增反而稍有降低.结论 脑出血大鼠血清白蛋白水平较假手术组显著下降;脑出血可引起小肠黏膜损害.rhGH可提高脑出血大鼠血清白蛋白水平,不论在脑出血早期还是后期均可不同程度减轻小肠黏膜损伤,后期改善更为显著.rhGH对小肠黏膜损伤的改善程度与血清白蛋白水平的升高程度呈正相关.

Effects of growth hormone on serum albumin levels and intestinal mucosal morphology in rats with intracerebral hemorrhage

Objecthe To equate the effects of recombinant human growth hormone (rhGH) on serum albumin lewis and intestinal mucosal morphology in rats with intracerebral hemorrhage. Methods A rat model of intracerebral hemorrhage was induced by autologous blood injection. Fifty-six SD rats were divided into sham-operation group (n =8), rhGH group (n =24; intraperitoneal injection of rhGH, 1 U/kg, once a day), and saline control group (n =24; intraperitoneal injection of equivalent normal saline, once a day). The rhGH and saline control groups were redivided into 1-, 7- and 14-day groups (n =8 in each group) after the procedure. The serum albumin concentration was detected at different time points in all groups. The changes of intestinal mucosal morphology were observed with Hematoxylin and Eosin (HE)staining and image analysis. Results Hie serum albumin lewis at all time points of intracerebral hemorrhage in the saline control group were all significantly lower than those in the sham-operation group (all P < 0. 01); The serum albumin level was increased gradually with the treatment process in the rhGH group, however, it was only significantly higher than the saline control group at day 14 (39.93 ±1.98 g/L νs. 37. 93 ±1.57 g/L) (P<0. 01). There were no significant differences between the rhGH group and the saline group in intestinal villus height and mucosal thickness at day 1 and 7 after intracerebral hemorrhage, however they were increased significantly at day 14 (P <0.01). The area of intestinal villi was reduced progressively at day 1, 7 and 14 after intracerebral hemorrhage, and with the treatment process the rhGH group was increased more progressively than the saline control group (P <0. 01). The depth of intestinal glands in the rhGH group was increased significantly than that in the saline control group (P <0. 01), but there was no significant difference at day 14; the density of glands in the rhGH group was significantly increased than that in the saline group at day 1 after intracerebral hemorrhage (P < 0. 01), and it was not increased significantly at day 7, however, it was not increased but decreased slightly at day 14. Conclusions The serum albumin level in rats with intracerebral hemorrhage was decreased significantly than that in the sham-operation group, and intracerebral hemorrhage could cause intestinal mucosal injury. rhGH increased the serum albumin level in rats with intracerebral hemorrhage. It might reduce intestinal mucosal injury to different degrees whether it was in the early or late intracerebral hemorrhage, and the late improvement was more significant. The improvement degree of rhGH on intestinal mucosal injury was positively correlated with the increased degrees of the serum albumin level.