| 羊水来源间充质细胞对于外周单核淋巴细胞增殖抑制作用的机制研究 |
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| 引用本文： | 吴小萍,李俊,张梦军,傅晓岚,姜曼,吴玉章. 羊水来源间充质细胞对于外周单核淋巴细胞增殖抑制作用的机制研究[J]. 免疫学杂志, 2010, 0(4) |
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| 作者姓名： | 吴小萍 李俊 张梦军 傅晓岚 姜曼 吴玉章 |
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| 作者单位： | 第三军医大学全军免疫研究所; |
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| 基金项目： | 国家自然科学基金青年科学基金资助项目(30900859) |
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| 摘 要： | 目的鉴定羊水来源间充质细胞(AF-MCs)并探讨其对于外周单核淋巴细胞(PBMC)增殖活性的影响及机制。方法通过选择性消化和单克隆培养分离及培养人源羊水细胞(AFCs),而后通过核型分析细胞来源和流式分析细胞表型鉴定其为间充质细胞(MCs)。另一方面,将鉴定得到的AF-MCs与PBMC共培养,检测其是否具有免疫抑制功能。运用流式分析CD4~+CD25~+Foxp3~+T细胞频率在共培养前后变化,探讨AF-MCs具有免疫抑制功能的机制。结果AF-MCs来自于胚胎发育过程中的脱落细胞,其具有与骨髓间充质干细胞(BM-MSCs)相似的表型,且其能上调CD4~+CD25~+Foxp3~+T细胞的频率而产生免疫抑制功能。结论AF-MCs具有与BM-MSCs类似的表型,并且在免疫调节方面与BM-MSCs类似。这为临床应用间充质干细胞提供了一个新的,安全的种子细胞来源。
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| 关 键 词： | 羊水 间充质细胞 外周单核淋巴细胞 免疫抑制 |
Inhibitory effects of amniotic fluid-derived mesenchymal cells on the proliferation of peripheral blood mononuclear cells and its mechanism |
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| WU Xiaoping,LI Jun,ZHANG Mengjun,FU Xiaolan,JIANG Man,WU Yuzhang Institute of Immunology,Third Military Medical University,Chong qing ,China. Inhibitory effects of amniotic fluid-derived mesenchymal cells on the proliferation of peripheral blood mononuclear cells and its mechanism[J]. Immunological Journal, 2010, 0(4) |
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| Authors: | WU Xiaoping LI Jun ZHANG Mengjun FU Xiaolan JIANG Man WU Yuzhang Institute of Immunology Third Military Medical University Chong qing China |
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| Affiliation: | WU Xiaoping,LI Jun,ZHANG Mengjun,FU Xiaolan,JIANG Man,WU Yuzhang Institute of Immunology,Third Military Medical University,Chong qing 40038,China |
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| Abstract: | In this study,we would identify the amniotic fluid-derived mesenchymal cells(AF-MCs) and explore the mechanism of the AF-MCs suppressing the proliferation of peripheral blood mononuclear cells(PBMC).We obtained and cultured the AF-MCs through the selective digestion and clone cultivation.Then FACS and Giemsa staining were employed to analyze the karyotype and phenotype of the AF-MCs for identifying the cells source and characters.And this analysis showed that AF-MCs were derived from the exfoliated cells du... |
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| Keywords: | Amniotic fluid Mesenchymal cells Peripheral blood mononuclear cells Immuno-suppression |
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