Bmi-1基因敲除导致小鼠皮肤老化

目的:探讨Bmi-1在抗皮肤衰老中的作用.方法:使用组织学,免疫组织化学和Western-blot的实验方法比较分析了同窝2周和4周龄Bmi-1基因敲除纯合子小鼠和野生型小鼠皮肤表型的差异.结果:Bmi-1纯合子小鼠出现皮肤变薄、角质层增厚、皮下脂肪和胶原减少等衰老表型.通过增殖细胞核抗原(PCNA)免疫组织化学染色.发现Bmi-1纯合子小鼠中PCNA阳性细胞较同窝野生型小鼠明显减少.Western-blot结果显示细胞周期依赖性激酶抑制因子p16、p19和p27在Bmi-1纯合子小鼠皮肤组织国都明显升高.结论:Bmi-1能够通过抑制细胞周期依赖性激酶抑制因子的表达.刺激皮肤细胞增殖而发挥抗皮肤衰老的作用.

Deletion of Bmi-1 gene leads to skin aging in mice

Objective:To investigate the role of Bmi-1 in preventing skin aging in mice. Methods:The phenotype of skin was compared between wild-type and Bmi-1 gene knock-out mice at 2 and 4 weeks of ages by histology, immunohistochemistry and Western-blot,respectively. Results:Bmi-1 gene knock-out mice displayed a premature aging phenotype in skin including thinner skin with hyperkeratosis of the epidermis, reduced collagen and subcutaneous fat deposition in skin matrix. Proliferating cell nuclear antigen immunopositi...