| 前交叉韧带损伤后关节腔内后交叉韧带中基质金属蛋白酶-II活性的研究 |
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| 引用本文： | 张瑾,罗自维,陈文琦,钱宇娜,汤振宇,薛茹月,王业全,宋国立,吕永钢,杨力. 前交叉韧带损伤后关节腔内后交叉韧带中基质金属蛋白酶-II活性的研究[J]. 医用生物力学, 2009, 24(3): 162-165 |
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| 作者姓名： | 张瑾 罗自维 陈文琦 钱宇娜 汤振宇 薛茹月 王业全 宋国立 吕永钢 杨力 |
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| 作者单位： | 重庆大学生物工程学院国家“111计划”基地;重庆大学生物工程学院国家“111计划”基地;重庆大学生物工程学院国家“111计划”基地;重庆大学生物工程学院国家“111计划”基地;重庆大学生物工程学院国家“111计划”基地;重庆大学生物工程学院国家“111计划”基地;重庆大学生物工程学院国家“111计划”基地;重庆大学生物工程学院国家“111计划”基地;Departments of Orthopaedics and Bioengineering, University of California;重庆大学生物工程学院国家“111计划”基地;重庆大学生物工程学院国家“111计划”基地 |
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| 基金项目： | 国家重点研究发展计划（2005CB522703）;高等学校学科创新引智计划（国家“111计划”）（B06023）;the OREF Award(USA);NIH AR45635(USA) |
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| 摘 要： | 目的 初步探索前交叉韧带(anterior cruciate ligament，ACL)损伤后后交叉韧带(posterior cruciate ligament，PCL)中基质金属蛋白酶(MMP-2)的表达量变化情况。方法 用本实验室已获专利的大鼠前交叉韧带瞬时扭转损伤装置将大鼠ACL损伤后在ex-vivo水平上用酶谱分析的方法检测PCL组织中所释放MMP-2的情况。结果 ACL损伤后的第1，2，3天后，PCL组织培养上清液中的MMP-2表达量呈时间依赖性递增趋势。结论 ACL急性损伤后, PCL组织释放大量MMP-2至关节液中。
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| 关 键 词： | 前交叉韧带；后交叉韧带；基质金属蛋白酶 |
| 收稿时间： | 2009-05-22 |
| 修稿时间： | 2009-05-22 |
Study about the activity of MMP-2 in intra-articular PCL after ACL injury |
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| ZHANG Jin,LUO Zi-wei,CHEN Wen-qi,QIAN Yu-n,TANG Zhen-yu,XUE Ru-yue,WANG Ye-quan,SONG Guo-li,LV Yong-gang and YANG Li. Study about the activity of MMP-2 in intra-articular PCL after ACL injury[J]. Journal of Medical Biomechanics, 2009, 24(3): 162-165 |
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| Authors: | ZHANG Jin LUO Zi-wei CHEN Wen-qi QIAN Yu-n TANG Zhen-yu XUE Ru-yue WANG Ye-quan SONG Guo-li LV Yong-gang YANG Li |
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| Affiliation: | 114' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University;114' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University;114' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University;114' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University;114' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University;114' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University;114' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University;114' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University;Departments of Orthopaedics and Bioengineering, University of California;114' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University;114' Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University |
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| Abstract: | Objective To detect the expression of MMP-2 of posterior cruciate ligament (PCL) tissue after anterior cruciate ligament (ACL) injury. Methods The rat knee injury apparatus designed by our lab was used to injure ACL. Then, the expression of MMP-2 in PCL was detected at ex-vivo level with zymography. Results The expression of MMP-2 in PCL cultured supernatant fluid increased in a time dependent way after ACL injury. Conclusion PCL contributed to the accumulation of MMP-2 in the joint fluid after acute ACL injury. |
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| Keywords: | Anterior cruciate ligament (ACL) Posterior cruciate ligament (PCL) Matrix metalloproteinase (MMP) |
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