穹窿海马伞切割侧海马对植入神经干细胞分化为神经元的影响

为观察成鼠神经干细胞移植入切割穹窿海马伞侧海马和正常侧海马后存活和分化为神经元的状况 ,用无血清培养和单细胞克隆技术获取成年 SD大鼠前脑室下带神经干细胞 ,进行 Brd U标记和扩增。切割 SD大鼠右侧穹窿海马伞 ,术后 2周将标记有 Brd U的神经干细胞植入双侧海马齿状回。 2月后 ,行 Nissl染色、Brd U免疫荧光、NF -2 0 0 / Brd U免疫荧光、β-tubulin- / Brd U免疫组织化学染色和 ACh E组织化学染色。结果发现 ,移植的神经干细胞在海马齿状回中存活并沿颗粒下层迁移 ,切割侧海马齿状回中有较多的 Nissl深染的大胞体神经元样细胞 ,而正常侧多为小胞体胶质样细胞。切割侧海马齿状回颗粒下层中见有数个NF-2 0 0 / Brd U、β-tubulin- / Brd U双标神经元和 ACh E阳性神经元 ,而正常侧海马中则未能见到。上述结果提示 ,移植到海马中的神经干细胞能存活、迁移 ,穹窿海马伞切割侧海马中某些物质的表达增强 ,可诱导植入其内的神经干细胞向神经元或 ACh E阳性神经元分化。

EFFECTS OF HIPPOCAMPUS OF FIMBRIA FORNIX TRANSECTED SIDE ON DIFFERENTIATION INTO NEURONS FROM THE GRAFTED NEURAL STEM CELLS

To investigate the survival and the neuronal differentiation of the neural stem cells(NSCs) transplanted into the bilateral hippocampus of adult rat with right fimbria fornix transected, the neural stem cells were obtained from the SVZ of adult rat brain using the serum-free culturing and single clone techniques. NSCs labeled by BrdU were transplanted into the bilateral hippocampus of the right fimbria fornix-transected brains. At the 2nd month after operation, Nissl staining, BrdU immunofluorescence, NF-200/BrdU immunofluorescence, β-tubulin-Ⅲ/BrdU double immunohistochemistry and AChE histochemistry were used to detect the survival and the neuronal differentiation of the BrdU-labeled cells in the hippocampus. The NF-200/BrdU double labeled neurons, the β-tubulin-Ⅲ/BrdU double labeled ones and the AChE positive ones were observed in the fimbria fornix transected hippocampus in all sections of the brains, but not in the contralateral ones. The above results suggest that some substances were expressed higher in the fimbria fornix transected hippocampus than that in the contralateral side, which can induce the grafted NSCs surviving and differentiating into neurons or AChE positive neurons.