苯乙酸对胶质瘤细胞C_6DNA合成的抑制作用

目的：观察诱导分化剂苯乙酸对胶质瘤细胞Ｃ６的诱导分化作用，并在分子水平上探讨其作用机理。方法：细胞计数和〔３Ｈ〕－ＴｄＲ掺入法检测细胞增殖，流式细胞术分析细胞周期。结果：苯乙酸对细胞增殖存在剂量（２５ｍｍｏｌ／Ｌ，５０ｍｍｏｌ／Ｌ）依赖性抑制；免疫细胞化学检测ＣＰＭ值降低，肿瘤细胞ＤＮＡ合成减少；细胞周期分析Ｇ０／Ｇ１期所占比例下降，Ｓ期相对升高。结论：苯乙酸可阻抑细胞增殖周期，使细胞周期增殖循环中止于Ｓ期中ＤＮＡ合成准备期Ｓ０期，ＤＮＡ合成减少，抑制细胞增殖。其作用机理为抑制细胞周期中Ｇ１期的ＲＮＡ和蛋白质合成

Inhibitory effects of phenylacetate on growth and differentiation of cultured glioma cess line C6

Objective:To investigate phenylacetate′s function of suppressing the growth of glioma C 6 cell and inducing differentitation and find the mechanism of this effect on molecular level.Methods:Cell proliferation was evaluated Using 3H -TdR and cell numeration,using a hemocytometer.Cell proliferation cycle was tested by flow cytometry (FCM).Results:NaPA (2.5 mmol/L and 5.0 mmol/L) exerted a marked inhibitory effect on cell Proliferation. Exposure to NaPA (2.5 mmol/L and 5.0 mmol/L for 5 days) resulted in a decrease of cells in G 0/G 1 Phase as being analyzed by flow cytometry.Conclusion:NaPA could exert a marked inhibitory effect on cell proliferation and glioma malignancy becomes lower.NaPA made cell cycle distribition on G 1 phase decreased and cell cycle stopped on S 0 phase (prepare period of DNA synthesis) by indreasing RNA and protein.