miR-302b在食管鳞癌细胞中的免疫学作用

目的研究miR-302b在食管鳞癌细胞中的免疫学作用。方法通过qRT-PCR检测ESCC癌症组织与癌旁组织中miR-302b的表达量,并在炎症刺激后观察食管鳞癌TE-10细胞的增殖与侵袭能力,以及miR-302b与癌症相关性炎症(cancer related inflammation,CRI)相关基因IRF2、ERBB4、CXCR4的表达量变化;通过转染改变miR-302b的表达水平,观察TE-10细胞的增殖与侵袭能力以及IRF2、ERBB4、CXCR4蛋白的表达量。结论 ESCC组织中miR-302b表达量显著减少;miR-302b可以通过减少肿瘤相关性验证CRI相关基因IRF2、ERBB4、CXCR4的表达量来抑制TE-10细胞的增殖与侵袭,从而抑制ESCC的发生与发展。

The expression and immunological effects of miR-302b in esophageal squamous cell carcinoma

This study was performed to investigate immunological function of miR-302 b in the esophageal squamous cell carcinoma(ESCC) tissues, and to investigate the clinical relevance of miR-302 b expression in ESCC.The expression of miR-302 b was detected by quantitative real time PCR(qRT-PCR) in 63 ESCC patients paired with NAT(non-tumor adjacent tissues). We also detected the cell proliferation and invasion in TE-10 cells and the levels of miR-302 b and CRI relative genes(IRF2/ERBB4/CXCR4) by inflammatory stimulation or transfected with the miRNA’s specific siRNA and mimics. Data showed that the level of miR-302 b was higher in ESCC tissues compared with NAT. In conclusion, miR-302 b could inhibit cell proliferation and invasion in TE-10 cells by inhibiting the expression of CRI genes(IRF2/ERBB4/CXCR4), thus prevents the development of ESCC.