三个半规管阻塞动物模型的建立

目的 旨在建立豚鼠单侧三个半规管阻塞的动物模型。方法 利用２０只豚鼠行单侧三个半规管阻塞，观察手术前后眼震电图、听性脑干反应（ａｕｄｉｔｏｒｙ ｂｒａｉｎｓｔｅｍ ｒｅｓｐｏｎｓｅｓ，ＡＢＲ）、畸变产物耳声发射（ｄｉｓｔｏｒｔｉｏｎ ｐｒｏｄｕｃｔ ｏｔｏａｃｏｕｓｔｉｃ ｅｍｉｓｓｉｏｎ，ＤＰＯＡＥ）及形态学的变化，非手术耳作对照。结果 豚鼠术后１ｄ出现自发性眼震，正弦摆动刺激单侧眼震反应消失，

Establishment of the animal model of triple semicircular canal occlusion]

OBJECTIVE: To establish the animal model of triple semicircular canal occlusion(TCO) in guinea pigs. METHODS: TCO was performed on 20 guinea pigs. Electronystagmography, auditory brainstem responses (ABR) and distortion product otoacoustic emissions(DPOAE) were recorded pre- and post-operatively. The non-operated ear was used as control. RESULTS: There was spontaneous nystagmus towards non-operated side on the 1st postoperative day and no nystagmus to stimuli of sinusoidal angular acceleration in operated side. On the 30th postoperative day, left and right nystagmus returned symmetrical. Pre-operation, ice-water stimulation induced normal symmetric nystagmus in both sides. On the 30th postoperative day, ice-water stimulation only induced normal nystagmus in non-operated ear and failed in operated one. ABR thresholds increased approximately 10 dB post-operation. There were no significant changes of the amplitudes of DPOAE before and after surgery. Histologic examination revealed complete occlusion in the three semicircular canals with newly formed bone and connective tissue. CONCLUSION: This study showed that the animal model of TCO was successfully established. These findings suggests that TCO might be a safe and simple procedure for the treatment of intractable peripheral vertigo.