帕金森病模型大鼠黑质多巴胺能神经元的氧化应激研究

目的　探索帕金森病模型大鼠黑质多巴胺能神经元的氧化应激发病机制。方法　通过立体定位仪 ,将 6-OHDA注入大鼠一侧纹状体内制备 PD模型 ,2周后观察动物的行为学改变 ,2个月后观察黑质纹状体等的病理形态学变化 ,检测模型组、假手术组和正常对照组的超氧化物歧化酶 (SOD)的活性 ,丙二醛 (MDA)、一氧化氮(NO)代谢产物 (NO x)的含量的变化。结果　成功 PD模型鼠有 2 2只。光镜下 HE染色示模型组右侧黑质的多巴胺神经元受损 ,数目减少。模型组右侧黑质的 SOD的含量下降 ,MDA及 NO x含量明显升高 ,与左侧、假手术组及正常对照组相比有显著差异 (P>0 .0 5)。结论　 6-OHDA纹状体内双靶点注射法是一种有效的制备 PD模型的方法。帕金森病大鼠模型黑质内 SOD活性下降 ,MDA、NO x含量升高 ,氧化应激在 PD的发病中起重要的作用

Oxidative stress of substantia nigral dopaminergic neuron in rat model of parkinson's disease

Objective To explore the oxidative stress pathogen esis of Parkinson's disease (PD) rat models in the neurons of substantia nigra.Methods 6-OHDA was stereotacticly injected into the right striatum of the rats at two sites in order to produce P D models.Observed the morphological alterations by striatal and substantia nigr al hematoxylin & eosin (HE) stain and compared the activity of superoxide dismutase (SOD),the quantities of malondialdehyde (MDA) and NO x(the metabolism products of nitric oxide) between the injuried tissue and no rmal tissues.Results In the model group of 30 rats,there were 22 successful PD models.Photomicrograph s of HE stain showed that the dopaminergic neurons in the right substantia nigra pars compacta (SNc) were severely lost,and the remaining neurons were atrophic.The level of SOD in the ri ght substantia nigra(SN) of the model group descended significantly,while the quantity of MDA and NO x increased obviously respectively compared with either the sham g roup or the normal control group (P<0.05).Conclusion It is rather effective method to make PD rat models using 6-OHDA injected into the striatium of one side at two target sites.In PD rats the acti vitis of SOD are descent,while the quantities of MDA and NO x are increas ed in the dopaminergic neurons of SN.Oxidative stress plays a key role in the pa thogenesis of PD.