还原型谷胱甘肽对六价铬诱导的L-02肝细胞毒性的保护作用

目的探讨还原型谷胱甘肽(GSH)对六价铬Cr(Ⅵ)]诱导的L-02肝细胞毒性的影响。方法分别设Cr(Ⅵ)、GSH单独作用组、联合作用组和空白对照组,采用MTT法检测细胞生存率的变化。结果在2、4、8、16、32和64μmol/LCr(Ⅵ)处理浓度,Cr(Ⅵ)对L-02肝细胞具有明显细胞毒性,细胞存活率与Cr(Ⅵ)处理浓度呈负相关(r=-0·910)。仅浓度为20μmol/L的GSH对Cr(Ⅵ)诱导的细胞毒性具有拮抗作用,Cr(Ⅵ)+GSH联合组的生存率与Cr(Ⅵ)不同浓度单独处理组比较差异均具有显著性(P<0·05)。结论适宜浓度的GSH可能对Cr(Ⅵ)诱导的L-02肝细胞毒性具有保护作用。

Protective effect of reduced glutathione on cytotoxicity induced by hexavalent chromium[ Cr(Ⅵ)] in L-02 hepatocyte

OBJECTIVE: To explore the effect of reduced glutathione (GSH) on cytotoxicity induced by hexavalent chromium (Cr(VI)) in L-02 hepatocyte. METHODS: The test has three groups:the groups of (Cr(VI), the groups of GSH, the groups of Cr (VI) and GSH. The survival rate of L-02 hepatocyte is assessed on the reductions of tetrazolium dye (MTT). RESULTS: Significant cytotoxicities of L-02 hepatocyte were observed at the concenations of 2,4,8,16,32 and 64 micromol/L Cr (VI). Concentration-dependent decrease in cell survival rate of Cr (VI)-treated L-02 hepatocytes were observed (r = -0.910) Protective effect on all concentrations of Cr(VI) (2 - 64 micromol/L) at the dose of 20 micromol/LGSH were found. CONCLUSION: The results demonstrated that proper concentrations of GSH could have protective effect on cytoxicity induced by Cr(VI) in L-02 hepatocyte. GSH of too low or too high concentrations don't has this effect.