miRNA-21与高血压并腔隙性梗死的相关性及预测价值

目的探讨微小RNA-21(miR-21)与高血压伴腔隙性梗死(腔梗)的相关性及诊断预测作用。方法将67例高血压患者根据是否合并腔梗分为高血压组31例和高血压并腔梗组36例,收集各项临床和实验室指标,采用实时荧光定量PCR(qRT-PCR)测量血清miR-21;绘制受试者工作曲线(ROC),评价miR-21对高血压并腔梗的诊断预测价值,通过Pearson相关分析了解miR-21与颈动脉IMT的相关性。结果高血压并腔梗组miR-21表达(2.08±0.61),显著高于高血压组(1.25±0.37,P0.05)。miR-21对高血压发生腔梗的ROC曲线下面积(AUC)为0.871,95%可信区间(CI)0.787~0.954(P0.05)。Pearson相关分析发现miR-21与颈动脉IMT呈正相关(R=0.763,P0.05)。结论 miR-21在高血压并腔隙性脑梗死患者中高表达,是高血压发生腔梗的独立预测因素,可作为高血压发生腔梗的一个简便、无创、有效的血清学诊断预测指标。

Relation and diagnosis of miRNA-21 for being used as a prediction target molecular marker of lacunar infarction with hypertension

Objective To investigate the expression and diagnosis of miRNA-21 for being used as a prediction target molecular marker of lacunar infarction with hypertension.Methods 67 cases of patients with hypertension was divided into hypertension group (31 cases) and hypertension with lacunar infarction group (36 cases).Then measured serum miR-21 using real-time fluorescent quantitative PCR (qRT-PCR) and collected the clinical and laboratory indexes;evaluated miR-21 predictive value to the diagnosis of hypertension with lacunar infarction through receiver operator characteristic curve (ROC).Then studied the pertinence of miR-21 and the carotid artery intima-media thickness (IMT) using Pearson correlation analysis.Results The expression of miR-21 in serum of hypertension with lacunar infarction patients was significantly higher than that of the hypertension group(2.08±0.61 VS.1.25±0.37,P<0.05.ROC analysis results showed that the area under the ROC(AUC) was 0.871,and 95% confidence interval(95% CI)was 0.787~0.954.Pearson correlation analysis found that miR-21 and carotid IMT were positively correlated (R=0.763,P<0.05).Conclusion The serum level of miR-21 in hypertension with lacunar infarction was significantly higher than that in hypertension patients.The miR-21 has certain predictive value of lacuncar infarction in hypertension.