Molecular identification of<Emphasis Type="Italic"> Trichinella spiralis</Emphasis> and<Emphasis Type="Italic"> Trichinella britovi</Emphasis> by diagnostic multiprimer large mitochondrial rRNA amplification |
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| Authors: | Email author" target="_blank">P?BorsukEmail author B?Moskwa K?Pastusiak W?Cabaj |
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| Institution: | (1) Department of Genetics, University of Warsaw, Pawinskiego 5A, 02–106 Warsaw, Poland;(2) Institute of Biochemistry and Biophysics of the Polish Academy of Science, Pawinskiego 5A, 02–106 Warsaw, Poland;(3) Witold Stefanski Institute of Parasitology of the Polish Academy of Science, Twarda 51/55, 00–818 Warsaw, Poland |
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| Abstract: | Trichinella parasites with different epidemiological features still occur in Europe and four species of genus Trichinella have been identified: T. spiralis, T. britovi , T. nativa and T. pseudospiralis. Until now, two of them, T. spiralis and T. britovi, have been identified in Poland. In our studies we selected sequence coding for large mitochondrial rRNA (mt LrDNA) as a genetic marker and developed a sensitive LrDNA multiprimer PCR assay allowing for rapid identification of T. spiralis and T. britovi, parasites present in wild and domestic animals in Poland. |
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