栽培藏红花与藏红花培养细胞的成分对比研究

目的 :比较人工栽培藏红花和藏红花培养细胞的主要成分种类以及含量的异同。方法 :利用傅立叶变换红外光谱法 (FTIR)和高效液相色谱法 (HPLC)对两类样品进行对比分析 ,借以考察藏红花细胞的培养效果。结果 :表明不同产地的藏红花的主要成分种类和含量差异显著 ,与藏红花相比 ,藏红花培养细胞中的代谢产物种类较少 ,但是其具有抗癌活性的藏红花素A(crocinA)的含量高于藏红花 2～3倍。结论 :藏红花培养细胞的主要成分种类与人工栽培藏红花基本相同 ,但其含量差异较大 ,培养细胞现只具有部分替代性。

Comparison on ingredients between cultured cell and cultivated saffron pistils

Objective: To compare the differences of a ctive ingredients between tissue cultured cells and cultiveated saffron pistils . Method: The experiment was carried out by the fourier t ransform infrared spectroscopy (FTIR) spectra and high performance liquid chroma tography (HPLC). Result: The data indicated that the spec ies and contents active ingredients in saffron pistils from different places wer e different. The species of active ingredients in tissue cultured cells are less than those in cultivated saffron pistils. However, the quantity of crocin A , which showed good anticancer effect, is 2-3 times more than that in cultivated saffron pistils. Conclusion: The active ingredients of t he tissue cultured cells are similar to those of saffron pistils , but their contents are different. Therefore, the tissue cultured cells can onl y be the part-substitutes of cultivated saffron pistils.