双波长分光光度法测定非等比例多组分制剂中的间苯二酚含量

目的:建立非等比例多组分制剂间苯二酚-水杨酸(5∶1)]中间苯二酚的含量测定方法。方法:采用双波长分光光度法,以282 nm(间苯二酚最大吸收波长)为测定波长、320 nm为参比波长,测定并计算间苯二酚的含量。结果:间苯二酚质量浓度在15⁴0μg/ml范围内与吸光度差值的线性关系良好(r=0.999 2);间苯二酚的平均回收率为100.32%,RSD=1.67%(n=3)。结论:本方法快速简便,结果准确。非等比例多组分制剂中,宜优先选择高浓度成分进行含量测定,并使得测定波长下成分之间的吸光度值差异尽可能小。

Content Determination of Dioxybenzene in Unequal-proportional Multi-component Preparation by Dual-wavelength Spectrophotometry

OBJECTIVE：To establish the method for the content determination of dioxybenzene in unequal-proportional multicomponent preparation dioxybenzene-salicylic acid（5 ∶ 1）]. METHODS：The content of dioxybenzene was determined and calculated by dual-wavelength spectrophotometry using 282 nm（maximum absorption wavelength of dioxybenzene）as detection wavelength and 320 nm as reference wavelength. RESULTS：The linear relationship of dioxybenzene in the range of 15-40 μg/ml to the absorbance difference was good（r=0.999 2）. Average recovery of dioxybenzene was 100.32%（RSD=1.67%,n=3）. CONCLUSIONS：The method is rapid,simple and accurate. The high concentration ingredient in the unequal-proportional multi-component preparation should be prior for the content determination. The detection wavelength should be selected to minimum the absorbance difference between ingredients as much as possible.