流动注射化学发光法测定人尿液中尿酸的含量

目的:建立一种快速、灵敏地测定人尿液中尿酸(UA)含量的新方法。方法:利用在碱性条件下,UA可以选择性地猝灭抗坏血酸与荧光素-表面活性剂-铜离子混合物产生的化学发光,结合流动注射技术,建立了流动注射化学发光法直接测定人尿液中UA含量的方法。反应试剂的浓度分别为氢氧化钠溶液0.5mol/L、荧光素5.0×10-4mol/L、铜离子1.0×10-4mol/L、溴化十六烷基三甲铵(CTMAB)5.0×10-3mol/L、抗坏血酸25mg/L,主泵(P1)、副泵(P2)流速均为2.5ml/min,光电倍增管负高压为800V。结果:UA的检测质量浓度在0.1²0mg/L范围内线性关系良好(r=0.999 0),检出限为3.3×10-2mg/L,平均加样回收率为99.63%,精密度RSD为1.2%(n=11)。结论:本方法操作简便、灵敏度高、选择性好,为尿液中UA含量测定提供了一种新方法。

Content Determination of Uric Acid by Flow Injection Chemiluminescence

OBJECTIVE： To establish a novel, rapid and sensitive method for the content determination of uric acid. METH- ODS： Chemiluminescence generated by ascorbic acid reacting with fluoreseein-copper-surfactant could be selectively quenched in the presence of uric acid in alkaline solution. Based on flow injection, flow injection chemiluminescence was developed to determine the contents of uric acid directly. The concentrations of reagents were sodium hydrate（0.5 mol/L）, fluorescein（5.0× 10-4 molfL）, Cu2＋ （1.0×10-4 mol/L）, CTMAB （5.0×10-3 mol/L）, ascorbic acid （25 mg/L）. The flow rates of main and donkey pumps were set at 2.5 ml/min with negative high voltage of 800 V. RESULTS： The linear range of uric acid was 0.1-20 mg/L （r=-0.999 0） with an average recovery of 99.63% （RSD=1.2% ,n=11）. The detection limit was 3.3×10-2 mg/L. CONCLUSIONS： This method is convenient, sensitive and selective, and can provide a new method for the content determination of uric acid in urine sample.