| 显色基质法定量检测盐酸多沙普仑注射液的细菌内毒素 |
| |
| 引用本文： | 彭燕,张玲莉,王宗春. 显色基质法定量检测盐酸多沙普仑注射液的细菌内毒素[J]. 中国药房, 2014, 0(41): 3909-3911 |
| |
| 作者姓名： | 彭燕 张玲莉 王宗春 |
| |
| 作者单位： | 武汉大学人民医院,武汉430060 |
| |
| 摘 要： | 目的:建立定量检测盐酸多沙普仑注射液中细菌内毒素的方法。方法:采用显色基质法,按照《中国药典》相关方法对不同批号的盐酸多沙普仑注射液分别进行干扰试验,确立其不干扰质量浓度。结果:盐酸多沙普仑注射液稀释至5 mg/ml时无干扰作用,回收率为50%100%。结论:显色基质法可用于盐酸多沙普仑注射液中细菌内毒素含量的定量检测。
|
| 关 键 词： | 盐酸多沙普仑注射液 显色基质法 细菌内毒素 干扰试验 |
Quantitative Determination of Bacterial Endotoxin in Doxapram Hydrochloride Injection by Chromogenic Substrate Method |
| |
| PENG Yan,ZHANG Ling-li,WANG Zong-chun. Quantitative Determination of Bacterial Endotoxin in Doxapram Hydrochloride Injection by Chromogenic Substrate Method[J]. China Pharmacy, 2014, 0(41): 3909-3911 |
| |
| Authors: | PENG Yan ZHANG Ling-li WANG Zong-chun |
| |
| Affiliation: | (Renming Hospital of Wuhan University, Wuhan 430060, China) |
| |
| Abstract: | OBJECTIVE：To set up a method for quantitative determination of bacterial endotoxin in Doxapram hydrochloride injection. METHODS：The interference test of different batches of Doxapram hydrochloride injection was conducted according to Chinese Pharmacopeia,so as to determine the noninterference concentration by chromogenic substrate method. RESULTS：The interference between the sample and the relative chromogenic substrate was eliminable when the sample was diluted to 5 mg/ml. Recovery rates were 50%-100%. CONCLUSIONS：The chromogenic substrate method is suitable for quantitative detection of bacterial endotoxin content in Doxapram hydrochloride injection. |
| |
| Keywords: | Doxapram hydrochloride injection Chromogenic substrate method Bacterial endotoxin Interference test |
| 本文献已被 维普 等数据库收录！ |
