萝卜硫素对饥饿诱导人脐静脉内皮细胞凋亡的抑制作用

目的:研究萝卜硫素抑制饥饿诱导人脐静脉内皮细胞(HUVECs)凋亡的作用。方法:以饥饿培养法诱导HUVECs凋亡。试验随机均分为正常对照(完全培养液)组、模型(模型培养液)组与萝卜硫素高、低浓度(10、5μmol/L)组。观察HUVECs形态;SRB法检测HUVECs存活率;Hoechst 33258染色法观察HUVECs的凋亡程度;吖啶橙染色和Western blot法观察HUVECs自噬水平的变化。结果:在饥饿条件下,萝卜硫素可以有效抑制饥饿HUVECs边缘发亮,脱离皿底的现象随时间延长而愈加明显;提高HUVECs的存活率;抑制HUVECs的凋亡,且具有剂量、时间依赖性;提高HUVECs的自噬水平。结论:萝卜硫素可能通过诱导自噬而起到抑制饥饿诱导HUVECs凋亡的作用。

Inhibitory Effects of Sulforaphen on Starvation-induced Apoptosis of Endothelial Cells

OBJECTIVE：To study the inhibitory effects of sulforaphen on starvation-induced apoptosis of endothelial cells.METHODS：The apoptosis of HUVECs was induced by starvation culture method. The apoptotic HUVECs were randomly divided into normal control group（complete culture solution）,model group（model culture solution）and sulforaphen high-concentration and low-concentration groups（10,5 μmol/L）. The morphology of HUVECs was observed,and the survival rate of HUVECs was detected by SRB method. The apoptosis of HUVECs was observed by Hoechst 33258 staining. The change of HUVECs autophagy was observed by acridine orange staining and Western blot assay. RESULTS：The sulforaphen could inhibit the phenomena that the edge of HUVECs became shiny and isolated from vessel bottom as time went on,enhanced the survival rate of HUVECs;inhibited the apoptosis of HUVECs in dose and time-dependant manner;improved HUVECs autophagy. CONCLUSIONS：The sulforaphen can inhibit the apoptosis of HUVECs by inducing HUVECs autophagy.