正交设计试验优选去甲斑蝥素在大鼠肝细胞中代谢的影响因素

目的:研究去甲斑蝥素(NCTD)在大鼠肝细胞中代谢的影响因素。方法:用MTT法检测NCTD对肝细胞的半数抑制浓度(IC50),以细胞接种密度、给药浓度、培养时间为考察因素,以培养体系中NCTD相对含量的峰面积为评价指标,采用气相色谱法检测NCTD的峰面积,正交设计试验筛选NCTD在肝细胞中代谢的影响因素。结果:NCTD对肝细胞的IC50为5.98μg/ml;在接种密度为75 000个/ml、给药质量浓度为6 g/ml、培养时间为48 h时,NCTD在肝细胞中代谢效率最高。结论:成功获得NCTD在肝细胞中代谢的最优条件。

Optimization of Influential Factors of the Metabolism of Norcantharidin in Rat Liver Cells by Orthogonal Test

OBJECTIVE：To study influential factors for the metabolism of norcantharidin（NCTD）in rat liver cells. METHODS：MTT assay was used to detect the IC50 of NCTD to liver cells. With cell vaccination density,administration concentration and incubation time as factors,using relative content of NCTD in culture system as index,GC was used to detect the peak area of NCTD,orthogonal test was adopted to optimize the influential factors for metabolism of NCTD in liver cells. RESULTS：The IC50 of NCTD to liver cells was 5.98 μ g/ml;when vaccination density was 75 000 piece/ml,administration concentration was 6 g/ml and culture time was 48 h,metabolic efficiency of NCTD in liver was the highest. CONCLUSIONS：This study obtains optimal metabolism conditions of NCTD in liver cells.