HPLC法同时测定双黄连颗粒中绿原酸、木犀草苷、连翘苷和黄芩苷的含量

目的:建立同时测定双黄连颗粒中绿原酸、木犀草苷、连翘苷和黄芩苷含量的方法。方法:采用高效液相色谱法。色谱柱为Eclipse XDB-C18,流动相为甲醇-0.4%磷酸溶液(梯度洗脱),流速为0.80 ml/min,检测波长为277 nm,柱温为30℃,进样量为10μl。结果:绿原酸、木犀草苷、连翘苷和黄芩苷检测质量浓度分别在3.91⁷8.3、0.8478.3、0.84¹6.7、1.8316.7、1.83³6.5、41.4736.5、41.47⁸29.4μg/ml范围内与各自峰面积积分值呈良好的线性关系(r=0.999 8、0.999 9、0.999 8、0.999 9);精密度、稳定性、重复性试验的RSD≤1.23%;平均加样回收率分别为100.59%、101.04%、100.90%、100.76%,RSD分别为1.50%、2.05%、1.95%、1.46%(n=9)。结论:该方法简便、准确、重复性好,可用于双黄连颗粒的质量控制。

Content Determination of Chlorogenic Acid,Luteolin, Forsythin and Baicalin in Shuanghuanglian Gran- ules by HPLC

OBJECTIVE： To establish the method for content determination of chlorogenic acid, luteolin, forsythin and ba- icalin in Shuanghuanglian granules. METHODS： HPLC method was adopted. Eclipse XDB-C18 column was used with mobile phase consisted of methanol-0.4%phosphoric acid solution （gradient elution） at the flow rate of 0.80 ml/min. The detection wavelength was set at 277 nm. The colmnn temperature was 30 % and the injection volume was 10 /al. RESULTS： The linear range were 3.91-78.3 μg/ml for chlorogenic acid（r=0.999 8）, 0.84-16.7 μg/ml for luteolin（r=0.999 9）, 1.83-36.5 μg/ml for forsythin（r= 0.999 8）and 41.47-829.4 μg/ml for baicalin（r=0.999 9）. RSDs of precision, Stability and reproducibility tests were all lower than 1.23%. The average recoveries were 100.59% （RSD=1.50%, n=9）, 101.04% （RSD=2.05%, n=9）, 100.90% （RSD= 1.95%, n=9） and 100.76% （RSD=1.46%, n=9）. CONCLUSIONS： The method is simple, accurate and reproducible, and can be used for the quality control of the granules.