消氮颗粒对腺嘌呤所致慢性肾衰模型大鼠的保护作用

目的:研究消氮颗粒对腺嘌呤所致慢性肾衰模型大鼠的保护作用。方法:灌胃腺嘌呤(200 mg/kg),每天1次,连续30 d,以复制大鼠慢性肾衰模型。60只Wistar大鼠随机均分为正常对照(等容纯化水)组、模型(等容纯化水)组、尿毒清颗粒(2.088 g/kg)组与消氮颗粒高、中、低剂量(3.956、1.977、0.989 g/kg)组,灌胃给药,每天1次,连续30 d。测定大鼠血肌酐(Scr)、尿素氮(BUN)、尿酸(UA)、电解质钾(K+)、钠(Na+)、钙(Ca2+)、磷(P3+)]、尿蛋白(PRO)、中性粒细胞(LEU)、尿比重(SG),光镜观察大鼠肾脏病理学变化。结果:与正常对照组比较,模型组大鼠血清Scr、BUN、UA、P3+水平升高,尿液PRO、LEU水平升高,SG降低,差异有统计学意义(P<0.01);大鼠肾脏呈灰白色,系膜区明显增宽,系膜基质明显增多,小管间质可见大量炎细胞浸润,血管壁增厚;与模型组比较,消氮颗粒高、中、低剂量组大鼠血清Scr、BUN、UA、P3+水平降低,尿液中PRO、LEU水平降低,SG升高,差异有统计学意义(P<0.01或P<0.05);大鼠肾组织病理情况有一定改善。结论:消氮颗粒对腺嘌呤所致慢性肾衰大鼠有改善作用。

Protective Effects of Xiaodan Granules on Renal Function of Rats with Chronic Renal Failure Induced by Adenine

OBJECTIVE：To study the protective effects of Xiaodan granules on renal function of rats with chronic renal failure（CRF）induced by adenine. METHORDS：60 Wistar rats were randomly divided into normal control group（constant volume of normal saline）,model group（constant volume of normal saline）,Niaoduqing granules group（2.088 g/kg）and Xiaodan granules high-dose,medium-dose and low-dose groups（3.956,1.977,0.989 g/kg）. They were given relevant medicines intragastrically,once a day,for consecutive 30 days. The renal failure model of rats was induced by intragastric administration of adenine（200 mg/kg）,once a day,for consecutive 30 days. The serum creatinine（Scr）,blood urea nitrogen（BUN）,uric acid（UA）,electrolyte K＋,Na＋,Ca2＋,P3＋,proteinuria（PRO）,neutrophils（LEU）and urine specific gravity（SG）were determined,and renal pathology was observed under light microscope. RESULTS：Compared with normal control group,the serum contents of Scr,BUN,UA and P3＋,and the contents of PRO and LEU in urine were increased in model group,while SG level was decreased;there was statistical significance（P〈0.01）. Renal tissue of rats was gray white,and intraglomerular mesangial region broadened significantly. The intraglomerular mesangial matrix increased significantly,and a large number of inflammatory cell infiltration and thickening of blood vessels were found in renal tubules and interstitium. Compared with model group,the serum contents of Scr,BUN,UA and P3＋,the contents of PRO and LEU in urine were decreased in Xiaodan granules high-dose,medium-dose and low-dose groups,while SG level was increased;there was statistical significance（P〈0.01,P〈0.05）. The symptom of renal pathology had been improved to certain extent. CONCLUSIONS：Xiaodan granules have definitely therapeutic effect on chronic renal failure of rats caused by adenine.