慢性自发性荨麻疹患者血清维生素D与基质金属蛋白酶9的检测

目的 探讨慢性自发性荨麻疹患者血清25羟维生素D［25（OH）D］与基质金属蛋白酶9（MMP?9）的水平及意义。方法 用高效液相色谱?串联质谱法检测56例慢性自发性荨麻疹患者和25例健康对照血清25（OH）D水平，用酶联免疫吸附试验检测血清MMP?9水平。采用独立样本t检验或秩和检验分析两组间指标水平的差异，Spearman秩相关分析评估各检测指标之间的相关性及其与病情的关系。结果 患者组中轻度19例，中度26例，重度11例。患者组血清25（OH）D水平为（21.74 ± 6.04） μg/L，健康对照组为（30.17 ± 2.21） μg/L；患者组MMP?9水平中位数（P25 ~ P75）为291.55（166.18，594.46） μg/L，健康对照组为138.46（94.27，233.12） μg/L，两组间25（OH）D、MMP?9水平差异均有统计学意义（P < 0.05）。患者组血清25（OH）D与MMP?9水平呈负相关（rs = -0.26，P < 0.05），MMP?9水平与症状严重程度呈正相关（rs = 0.27，P < 0.05），25（OH）D水平与症状严重程度无明显相关关系（rs = -0.20，P > 0.05）。结论 维生素D、MMP?9在慢性自发性荨麻疹的发病过程中起一定作用。

Detection of serum levels of vitamin D and matrix metalloproteinase 9 in patients with chronic spontaneous urticaria

Yin Jiawen, Yang Wenlin Department of Dermatology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China Corresponding author: Yang Wenlin, Email: yangwenlin@21cn.com 【Abstract】 Objective To detect serum levels of 25-hydroxyvitamin D （25［OH］D） and matrix metalloproteinase 9 （MMP-9） in patients with chronic spontaneous urticaria （CSU）, and to explore their significance. Methods Totally, 56 patients with CSU and 25 healthy controls were enrolled into this study. High-performance liquid chromatography tandem-mass spectrometry （LC-MS/MS） and enzyme-linked immunosorbent assay （ELISA） were performed to measure serum levels of 25（OH）D and MMP-9 respectively in these subjects. Statistical analysis was carried out by two independent samples t-test or Wilcoxon rank sum test for comparisons of the above indexes between the two groups, and by Spearman rank correlation analysis for assessment of relationships between the serum levels of 25（OH）D and MMP-9 as well as between the two indexes and disease severity. Results Of the 56 patients, 19 were diagnosed with mild CSU, 26 with moderate CSU and 11 with severe CSU. Compared with the control group, the patient group showed significantly lower serum levels of 25（OH）D （21.74 ± 6.04 vs. 30.17 ± 2.21 μg/L, P < 0.05）, but significantly higher serum levels of MMP-9 （Median ［P25 - P75］: 291.55 ［166.18 - 594.46］ vs. 138.46 ［94.27 -233.12］ μg/L, P < 0.05）. In the patient group, the serum level of 25（OH）D was negatively correlated with that of MMP-9 （rs = －0.26, P < 0.05）, but uncorrelated with the severity of CSU （rs = －0.20, P > 0.05）; the serum level of MMP-9 was positively correlated with the severity of CSU （rs = 0.27, P < 0.05）. Conclusion Vitamin D and MMP-9 may play a role in the occurrence of CSU.