CJ亚单位疫苗诱导淋巴细胞活化的实验研究

目的：探讨空肠弯曲菌（camplobacter jejuni，CJ）亚单位脂质体佐剂疫苗免疫新西兰兔后诱导T、B细胞活化情况。方法：应用FACS、MTF法分别对CJ疫苗免疫新西兰兔外周血中CD4^＋、CD8^＋T淋巴细胞数的百分比及脾淋巴细胞转化率进行观察。结果：CJ疫苗免疫新西兰兔后外周血中CD4^＋细胞数量有明显的升高，CD8^＋细胞数量有一定程度的升高；脾淋巴细胞对PHA、SPA、特异性抗原（CJAg）均有明显的反应性。结论：CJ疫苗免疫动物可诱导淋巴细胞的活化，提示CJ疫苗可引起机体免疫细胞的应答效应。

Study on activation of lymphocyte induced by CJ vaccine

Objective:To study the effects on activation of lymphocytes, which induced by subunit liposomal adjuvant vaccine of Campylobacter jejuni(CJ), New Zealand rabbits were immunized to provide scientific experimental basis for immune mechanism.Methods: FACS,MTT were used to detect the number of CD_4~+ and CD_8~+ T cell in peripheral blood lymphocytes of rabbits vaccinated, the stimulation index (SI) of the spleen lymphocytes transformation with cationic or anionic liposomal adjuvant vaccine of CJ.Results: The number of CD_4~+ T cell in peripheral blood lymphocytes increased obviously, CD_8~+ T cell also increased at a certain extent, the spleen lymphocytes responsed to PHA,SPA or CJAg after vaccinated.Conclusion:The CJ vaccines can induce activation of lymphocytes response.The results indicate that the immune cells were induced the responsion effectively by the vaccines.