牛蒡子苷元对人肝癌细胞HepG2缝隙连接蛋白表达的影响 |
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引用本文: | 袁国卿.牛蒡子苷元对人肝癌细胞HepG2缝隙连接蛋白表达的影响[J].中医临床研究,2014(22):16-17. |
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作者姓名: | 袁国卿 |
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作者单位: | 南阳医学高等专科学校; |
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摘 要: | 目的:观察牛蒡子苷元在体外对人肝癌细胞HepG2缝隙连接蛋白(Cx)表达的影响。方法:采用Western blot 技术检测牛蒡子苷元对人肝癌细胞HepG2缝隙连接蛋白(Cx26/32/43)的表达。结果:Western blot 检测,用国产多克隆Cx一抗和进口单克隆Cx一抗的实验均显示:牛蒡子苷元等能明显提高人肝癌细胞HepG2Cx32的表达,且有时间、浓度依赖性,氧化剂H2O2能抑制牛蒡子苷元此作用;但牛蒡子苷元对HepG2的Cx43表达影响24h不明显,48h能明显提高Cx43表达。结论:牛蒡子苷元能提高人肝癌细胞Cx表达,增强细胞间缝隙连接功能。
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关 键 词: | 牛蒡子苷元 肝癌 缝隙连接蛋白 |
Intervention of Niubangzi aglycone on expression of HepG2Cx |
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Abstract: | Objective: To observe intervention of Niubangzi aglycone on expression of HepG2Cx. Methods: Intervention of Niubangzi aglycone on expression of HepG2Cx26/32/43 was tested by Western blot. Results:Experiment of domestic polyclonal anti-Cx antibody and imported monoclonal anti-Cx antibody appeared that Niubangzi aglycone could obviously increase expression of HepG2Cx32 with time and concentration-dependent, oxidant H2O2 could inhibit the effect of Niubangzi aglycone; Niubangzi aglycone could not obviously affect expression of HepG2Cx43 within 24h, Niubangzi aglycone markedly improved expression of HepG2Cx43 within 48h with concentration-dependent. Conclusion:Niubangzi aglycone improved HepG2Cx expression and GJIC function. |
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Keywords: | Niubangzi aglycone Liver cancer Connexin |
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