抗中笥粒细胞胞浆抗体对溃疡性结肠炎的诊断价值

目的 探讨抗中性粒细胞胞浆抗体（ＡＮＣＡ）对溃疡性结肠炎（ＵＣ）的诊断价值。方法 应用间接免疫荧光法（ＩＩＦ）、酶联免疫吸附法（ＥＬＩＳＡ）和免疫印法（Ｗｅｓｔｅｍ ｂｌｏｔ）分别对ＵＣ患者（５８例）、非ＵＣ患者患者（４３例）及健康献血员（５８例）进行血清ＡＮＣＡ检测。结果 ＡＮＣＡ对ＵＣ诊断的敏感性为３７．９３％,特异性为１００％。ＵＣ患者中依据病情分为轻、中、重度组,ＡＮＣＡ的阳性率分别为１７

The diagnostic significance of antineutrophil cytoplasmic antibodies in ulcerative colitis

OBJECTIVE: To inquire into the diagnostic significance of antineutrophil cytoplasmic antibodies (ANCA) in ulcerative colitis (UC). METHODS: Serum ANCA from 58 UC patients, 43 non-UC patients and 58 healthy donors were detected with indirect immunofluorescence (IIF), enzyme linked immunosorbent assay (ELISA)and Western blot. RESULTS: The sensitivity and specificity of the presence of ANCA in the diagnosis of UC were 37.93% and 100% respectively. In the group of UC, patients with mild, moderate and severe clinical manifestations had positive ANCA rates of 17.65%, 41.67% and 52.94% respectively. The occurrence rates of mucosal vasculitis and grade III-V mucosal inflammation were 78.95% and 78.95% respectively in ANCA-positive group, but 37.04% and 44.44% in ANCA-negative group. The levels of the binding of the five kinds of ANCA antigens i.e. myeloperoxidase (MPO), bactericidal/permeability increasing protein (BPI), lactoferrin (LF), cathepsin G (CG) and proteinase-3 (PR-3) with the serum of UC patients were 13.99%, 13.79%, 10.34% 10.34% and 8.62% respectively. Specific protein strips were observed in 48.28% of the UC patients with Western blot technique, 47,000 polypeptide being the most common and making up 22.41% of them. CONCLUSION: The detection of ANCA was an auxiliary method for the diagnosis of UC. At present, UC-associated target antigens were not yet clarified, 47,000 polypeptide may be one of them. ANCA may participate in the pathogenesis of UC.