白细胞介素-6及其受体在系统性红斑狼疮患者外周血中的表达

目的 了解系统性红斑狼疮（ＳＬＥ）患者血清白细胞介素－６（ＩＬ－６）和可溶笥白细胞介素－６受体（ｓＩＬ－６Ｒ水平及其与临床活动性指标的相关性,并初步探讨其ＩＬ－６信号传导途径。方法 采用ＭＴＴ比色法和酶联免疫吸附实验（ＥＬＩＳＡ）法分别检测１６例ＳＬＥ（９例活动,７例非活动）、１７例活动期类风湿关节炎（ＲＡ）患者及２９例正常人血清ＩＬ－６和ｓＩＬ－６Ｒ水平。并在体外用ＩＬ－６刺激分离单个核细胞后,

Expression of interleukin-6 and its receptors in peripheral blood of patients with systemic lupus erythematosus

Objective To investigate the level of interleukin 6 (IL 6) and soluble IL 6 receptor (sIL 6R) in serum of patients with systemic lupus erythematosus (SLE) and its correlation with disease activity and to explore IL 6 signal transduction pathways in SLE patients. Methods By using MTT colorimetry and ELISA, the levels of IL 6 and sIL 6R in serum from 16 patients with SLE (9 active, 7 inactive), 17 with active rheumatoid arthritis (RA) and 29 controls were measured. The expressions of acute phase reaction factor (APRF) in peripheral blood mononuclear cells (PBMCs) were also detected by using eletrophoresis mobility shift assays (EMSA) after the PBMCs were isolated and then stimulated by IL 6. Results Serum IL 6 and sIL 6R levels in active SLE patients were significantly higher than those in normal controls ( P <0.01), but were significantly lower than those in active RA patients ( P <0.01). Serum IL 6 rather than sIL 6R level in active SLE patients was significantly higher than that in inactive ones ( P <0.01). Serum IL 6 was correlated positively with the anti dsDNA antibody ( r =0 658, P <0.01), but not with CRP ( r =0.041, P >0.05). There were no expression of APRF in PMNC from SLE patients and 8 normal controls, whereas PMNC from 11 RA patients expressed APRF activity (11/17). Conclusion The level of IL 6 is a useful parameter for monitoring disease activity of SLE. In active SLE, no significant elevation of serum sIL 6R and no expression of APRF in PMBCs may be responsible for mild CRP elevation.