肝癌细胞清除乙型肝炎病毒的非免疫机制

目的 试图用肝癌细胞体外培养试验,推测肝细胞表达ＣＤ９５配体是否能自身通过凋亡来清除乙型肝炎病毒。方法 诱导ＨｅｐＧ２细胞表达ＣＤ９５Ｌ,与转染ＨＢＶ,表达ＣＤＳ９５的ＨｅｐＧ２．２．１５细胞共培养,以荧光显微镜和流式细胞仪检测后者的凋亡。结果 表达ＣＤ９５Ｌ的ＨｅｐＧ２细胞杀伤表达ＣＤ９５的ＨｅｐＧ２．２．１５细胞,２４小时凋亡１６．５％,４８小时凋亡４３．０％,这一作用可用抗－ＣＤ９５阻断。

Clearance of intrahepatic hepatitis B virus by a nonimmune mechanism

Objective The clearance of intrahepatic hepatitis B virus(HBV) is mainly by the cytotoxicity of immune cells with ways including CD95ligand/CD95 induction. We found that the liver cells do express CD95 ligand(CD95L), a cytotoxic cellsurface molecule. This paper studied the mechanism and implications of the hepatocytes themselves to clear the virus. Methods The HepG2 cells with induced CD95L and the HepG2.2.15 cells with constitutive CD95 expression were coincubated. The apoptotic cells were examined under fluorescene microscope and with flow cytometry. Results In chronic hepatitis B intrahepatic expressions of CD95L and/or CD95 were detected at both mRNA and protein level. Methotrexate induced CD95L expression of HepG2 cell. The HepG2 cells with CD95L could kill the HepG2.2.15 cells with CD95 molecule with apoptotic rates of 16.5% at 24h and 43.0% at 48h. This apoptosis effect could be blocked by using antiCD95L antibody. Conclusion The hepatocyte might induce apoptosis mutually and clear the virus themselves with a nonimmune mechanism.