支气管哮喘患者记忆性T淋巴细胞对B淋巴细胞产生IgE作用的研究

目的 探讨记忆性（ＣＤ４＾＋ＣＤ４５ＲＯ＾＋）Ｔ淋巴细胞在支气管哮喘的发病中的作用。方法 分别分离出支气管哮喘病人及健康对照的ＣＤ４＾＋ＣＤ４５ＲＯ＾＋Ｔ淋巴细胞亚群，并将其与各自的Ｂ淋巴细胞共同培养，设定刺激组（美洲商陆有丝分裂原）及非刺激组，测定培养上清液中ＩｇＥ的含量。结果 哮喘病人自然状态的ＣＤ４＾＋ＣＤ４５ＲＯ＾＋Ｔ淋巴细胞对Ｂ淋巴细胞产生ＩｇＥ有正向促进作用，但有接受美洲商陆有丝分裂原

The effect of memory T lymphocytes on IgE production by B lymphocytes in patients with bronchial asthma

Objective To study the role of CD 4CD 45 RO T lymphocytes in the pathogenesis of bronchial asthma. Methods CD 4CD 45 RO T lymphocyte subsets were isolated from peripheral blood of patients with bronchial asthma and healthy donors. Then they were cultured with self B lymphocytes and designated as pokeweed mitogen (PWM) stimulated and non stimulated group. Secreted IgE was measured in the supernatant. Results CD 4CD 45 RO T lymphocytes had a positive role on IgE production by B lymphocytes from patients of bronchial asthma in non stimulated group, but the positive role did not exit in the presence of PWM. The effect of activated and non activated CD 4CD 45 RO T lymphocytes from patients with bronchial asthma on the secretion of IgE by B lymphocytes were turther investigated. Conclusion The function of activated CD 4CD 45 RO T lymphocytes returned to normal level. It is suggested that CD 4CD 45 RO T lymphocytes have adverse effects on IgE production in patients with bronchial asthma; which may be related to the pothogenesis of asthma.