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Expression profiles of 10 circadian clock genes in human peripheral blood mononuclear cells |
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| Authors: | Hiroaki Kusanagi Akiko Hida Kohtoku Satoh Masaru Echizenya Tetsuo Shimizu Julie S. Pendergast Shin Yamazaki Kazuo Mishima |
| Affiliation: | aDivision of Neuropsychiatry, Department of Neuro and Locomotor Science, Akita University School of Medicine, Akita, Japan bDepartment of Psychophysiology, National Institute of Mental Health, National Center of Neurology & Psychiatry, Japan cDepartment of Biological Sciences, Vanderbilt University, Nashville, USA |
| Abstract: | The circadian clock system regulates daily rhythms of physiology and behavior. The mammalian master clock in the suprachiasmatic nuclei orchestrates these biological rhythms in peripheral tissues. Since blood is the most accessible tissue source, we sought to dissect the human circadian clock system by characterizing clock gene expression in human peripheral blood mononuclear cells (PBMCs) isolated from eight young, healthy subjects. By evaluating the temporal expression profiles of 10 circadian clock genes, we found that Period 1 (Per1), Per2, and Per3 are rhythmically expressed in human blood samples. Our results suggest that evaluating the rhythmic expression of human Per genes could reveal an individual's circadian phenotype. |
| Keywords: | Circadian Human peripheral blood mononuclear cells (PBMCs) Clock genes Expression profiles Period (Per) |
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